PXD015700

PXD015700 is an original dataset announced via ProteomeXchange.

Title	Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs
Description	The objective of this study was to assess whether Methylocystis sp. strain SC2, as a representative for Methylocystis spp., can utilize hydrogen to optimize the biomass yield by mixed utilization of CH4 and H2, rather than CH4 as the sole source of energy. Thus, we aimed to show that, in the presence of H2, CH4 will primarily be used for synthesis of cell carbon and increased biomass/protein yield. In particular, we intended to explore those CH4/O2 ratios, which maximize the effect of hydrogen addition on the biomass yield and proteome reconstruction of strain SC2. To achieve our goals, we combined hydrogen-based growth experiments with our recently optimized proteomics workflow.
HostingRepository	PRIDE
AnnounceDate	2020-06-03
AnnouncementXML	Submission_2020-06-03_01:22:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015700
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Anna Hakobyan
SpeciesList	scientific name: Methylocystis sp. SC2; NCBI TaxID: 187303;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-04 03:17:34	ID requested
⏵ 1	2020-06-03 01:22:26	announced

Hakobyan A, Zhu J, Glatter T, Paczia N, Liesack W, Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs. Metab Eng, 61():181-196(2020) [pubmed]

submitter keyword: Knallgas bacteria, hydogenase, methanotrophs, Methylocystis, proteomics, metabolic model

PD Dr. Werner Liesack
contact affiliation	Max Planck Institute for Terrestrial Microbiology, Research Group Methanotrophic Bacteria and Environmental Genomics/Transcriptomics, Marburg, Germany
contact email	liesack@mpi-marburg.mpg.de
lab head
Anna Hakobyan
contact affiliation	Max Planck Institute for Terrestrial Microbiology
contact email	hakobyan@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015700
     1. Label: PRIDE project
     2. Name: Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs