PXD015692

PXD015692 is an original dataset announced via ProteomeXchange.

Title	Characterizing the interactome of human Arginyl-tRNA Synthethase
Description	Prior work revealed nuclear-localized aminoacyl-tRNA synthetases (aaRSs) that checked newly synthesized tRNAs for charging before export to the cytoplasm. To reveal other functions, we sought to identify nuclear proteins that interact with arginyl-tRNA synthetase (ArgRS) in the nucleus. Serine/Arginine Repetitive Matrix Protein 2 (SRRM2), which is stored with RNA splicing apparatus components in nuclear speckle condensates, was found as a consistent interaction partner that co localized with SRRM2 ArgRS in nuclear speckles. Dynamic photo-bleaching experiments showed that, consistent with condensate properties, SRRM2 has a fluctuating appearance in speckles. Knock down of ArgRS impeded SRRM2 speckle trafficking and, coincidently, altered splicing processing of pre-mRNA transcripts. Among the altered spliced variants, those of tRNA synthetase family members were prominent. Thus, nuclear ArgRS shapes the dynamics of a protein in class of nuclear condensates. Also, the work expands the repertoire of nuclear tRNA synthetase roles to include regulation of RNA splicing, including of aaRS family member transcripts.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:22.667.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015692
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Haissi Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-04 01:17:13	ID requested
1	2023-04-14 13:04:32	announced
⏵ 2	2023-11-14 08:50:23	announced	2023-11-14: Updated project metadata.

10.6019/PXD015692;

submitter keyword: arginine, tRNA syntethase,tRNA

Paul Schimmel
contact affiliation	Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA Department of Molecular Medicine, The Scripps Research Institute, Jupiter, FL 33458, USA
contact email	schimmel@scripps.edu
lab head
Haissi Cui
contact affiliation	Scripps Research
contact email	hacui@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD015692

PRIDE project URI

• PRIDE
  1. PXD015692
     1. Label: PRIDE project
     2. Name: Characterizing the interactome of human Arginyl-tRNA Synthethase