PXD015689

PXD015689 is an original dataset announced via ProteomeXchange.

Title	Use of condensed tannins to improve dietary protein utilization in ruminant diets: effects on lamb tissue proteome
Description	Cistus ladanifer L. is a common shrub endemic to the Mediterranean that is highly concentrated in condensed tannins (CT). CT form complexes with dietary protein that resist microbial degradation in the rumen, which enhances dietary protein utilization in ruminant diets. The objective of this study was to evaluate the utilization of CT in the diet of lambs on the proteomes of muscle, hepatic and adipose tissues. Twenty-four white merino ram lambs were divided in three groups (n=8) fed on different diets: control (16% crude protein - CP), reduced protein (12% CP) and reduced protein treated with CT extract. At the end of the trial, lambs were slaughtered and the longissimus lumborum muscle, hepatic and peri-renal adipose tissues sampled. A two-way approach was used for shotgun proteomic analysis: 2D-DIGE (gel-based) and nanoLC-MS (gel-free). In the muscle, control lambs had lower abundance proteins that partake in the glycolysis pathway. With dietary 12 % CP, lambs had higher abundance of Fe-carrying proteins in the hepatic tissue. Lambs with dietary CT had higher abundance of hepatic flavin reductase. In the adipose tissue, control lambs had lower abundance of fatty-acid synthase. In conclusion, CT inclusion influences specific pathways in lamb tissues.
HostingRepository	PRIDE
AnnounceDate	2021-02-16
AnnouncementXML	Submission_2021-02-16_02:28:09.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015689
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	Trp->Kynurenin; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-03 03:32:24	ID requested
1	2021-02-16 01:48:49	announced
⏵ 2	2021-02-16 02:28:10	announced	2021-02-16: Updated project metadata.

Ribeiro DM, Planchon S, Leclercq CC, Dentinho MTP, Bessa RJB, Santos-Silva J, Paulos K, Jer, ó, nimo E, Renaut J, Almeida AM, The effects of improving low dietary protein utilization on the proteome of lamb tissues. J Proteomics, 223():103798(2020) [pubmed]

10.1016/J.JPROT.2020.103798;

submitter keyword: condensed tannins, LC-MS, 2D-DIGE, proteome, hepatic, peri-renal adipose tissues, longissimus lumborum muscle

Celine LECLERCQ
contact affiliation	LIST-ERIN-ERTP-Green Tech Integrative Biology platform Belvaux, LUXEMBOURG
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-GreenTech platform
contact email	celine.leclercq@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015689
     1. Label: PRIDE project
     2. Name: Use of condensed tannins to improve dietary protein utilization in ruminant diets: effects on lamb tissue proteome