PXD015686

PXD015686 is an original dataset announced via ProteomeXchange.

Title	Min protein in vitro protein expression kinetics
Description	The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves on a planar membrane and into various oscillatory patterns in closed compartments. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to drive higher-order cellular functions. However, to convey hereditary information in a synthetic cell, the Min system must be encoded in a genomic DNA that can be copied, transcribed and translated. Here, the MinD and MinE proteins are de novo synthesized from their genes inside liposomes. Dynamic protein patterns and liposome shape deformation accompanying Min protein recruitment to the membrane are observed. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro.
HostingRepository	PanoramaPublic
AnnounceDate	2019-11-11
AnnouncementXML	Submission_2019-11-11_05:58:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Foschepoth
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Carbamidomethyl
Instrument	6460 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 16:16:50	ID requested
⏵ 1	2019-11-11 05:58:47	announced

Godino E, L, ó, pez JN, Foschepoth D, Cleij C, Doerr A, Castell, à CF, Danelon C, De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns. Nat Commun, 10(1):4969(2019) [pubmed]

submitter keyword: Min system, CFPS, in vitro gene expression, MRM, SRM

Christophe Danelon
contact affiliation	TU Delft
contact email	C.J.A.Danelon@tudelft.nl
lab head
David Foschepoth
contact affiliation	TU Delft
contact email	d.j.foschepoth@tudelft.nl
dataset submitter

Panorama Public dataset URI