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PXD015686

PXD015686 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMin protein in vitro protein expression kinetics
DescriptionThe Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves on a planar membrane and into various oscillatory patterns in closed compartments. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to drive higher-order cellular functions. However, to convey hereditary information in a synthetic cell, the Min system must be encoded in a genomic DNA that can be copied, transcribed and translated. Here, the MinD and MinE proteins are de novo synthesized from their genes inside liposomes. Dynamic protein patterns and liposome shape deformation accompanying Min protein recruitment to the membrane are observed. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro.
HostingRepositoryPanoramaPublic
AnnounceDate2019-11-11
AnnouncementXMLSubmission_2019-11-11_05:58:45.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Foschepoth
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListCarbamidomethyl
Instrument6460 Triple Quadrupole LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-02 16:16:50ID requested
12019-11-11 05:58:47announced
Publication List
Godino E, Lˇpez JN, Foschepoth D, Cleij C, Doerr A, CastellÓ CF, Danelon C, De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns. Nat Commun, 10(1):4969(2019) [pubmed]
Keyword List
submitter keyword: Min system, CFPS, in vitro gene expression, MRM, SRM
Contact List
Christophe Danelon
contact affiliationTU Delft
contact emailC.J.A.Danelon@tudelft.nl
lab head
David Foschepoth
contact affiliationTU Delft
contact emaild.j.foschepoth@tudelft.nl
dataset submitter
Full Dataset Link List
Panorama Public dataset URI