PXD015680

PXD015680 is an original dataset announced via ProteomeXchange.

Title	Dissecting the antibacterial activity of Oxadiazolone-core derivatives against Mycobacterium abscessus
Description	Mycobacterium abscessus is nowadays under the spotlight of the scientific community. This pathogenic mycobacteria is indeed responsible for a wide spectrum of infections involving mostly pulmonary infections in patients with cystic fibrosis. M. abscessus is intrinsically resistant to a broad range of antibiotics, including most antitubercular drugs, and is considered the most pathogenic and chemotherapy-resistant rapidly growing mycobacterium. Consequently, with very limited treatment options, the development of new therapeutic approaches to fight this pathogen are urgently needed. In this context, 19 oxadiazolone (OX) derivatives have been investigated for their antibacterial activity against both the rough (R) and smooth (S) variants of M. abscessus. Several OXs were active against extracellular M. abscessus growth with moderated minimal inhibitory concentrations (MIC), or intracellularly by inhibiting M. abscessus growth inside infected macrophages with MIC values similar to those of imipenem. Such promising results prompted us to identify the potential target enzymes of the sole extra and intracellular inhibitor of M. abscessus growth, i.e., iBpPPOX via activity-based protein profiling combined with mass spectrometry. This approach led to the identification of 21 potential protein candidates being mostly involved in M. abscessus lipid metabolism and/or in cell wall biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2020-08-14
AnnouncementXML	Submission_2020-09-27_23:53:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Mycobacterium abscessus; NCBI TaxID: 36809;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 05:08:01	ID requested
1	2020-08-14 02:11:38	announced
⏵ 2	2020-09-27 23:53:35	announced	2020-09-28: Updated publication reference for PubMed record(s): 32946441.

Madani A, Mallick I, Guy A, Crauste C, Durand T, Fourquet P, Audebert S, Camoin L, Canaan S, Cavalier JF, Dissecting the antibacterial activity of oxadiazolone-core derivatives against Mycobacterium abscessus. PLoS One, 15(9):e0238178(2020) [pubmed]

submitter keyword: Drug susceptibility

intracellular activity

activity based-protein profiling

proteomic analysis

Luc Camoin
contact affiliation	Aix Marseille Univ, Inserm, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Protéomique & Aix Marseille Univ, CNRS, LISM, Institut de Microbiologie de la Méditerranée
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015680
     1. Label: PRIDE project
     2. Name: Dissecting the antibacterial activity of Oxadiazolone-core derivatives against Mycobacterium abscessus