PXD015677

PXD015677 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SiTrap proteomics profiling of renal normal/tumor tissue pairs
Description	These are supporting RAW files for 'Detergent-free simultaneous sample preparation method for proteomics and metabolomics' SiTrap method manuscript. Frozen renal tissue from three matched clear cell renal carcinoma (G2 pT3a, G2 pT1b, G1 pT2) /adjacent normal sample pairs were obtained from The Leeds Multidisciplinary Research Tissue Bank. Approximately 1 cm2 sections with a thickness of 10 um were lysed and processed according to SiTrap protocol using SiTrap cellulose tips. The SiTrap load was normalized by protein concentration. 50 ug of protein was loaded. The collected flow-through fraction, devoid of proteins, was dried down for targeted metabolomics analysis. The captured protein fraction was reduced and alkylated in situ and digested with trypsin according to SiTrap protocol. The resulting peptides were concentrated for proteomics analysis.
HostingRepository	MassIVE
AnnounceDate	2019-10-14
AnnouncementXML	Submission_2019-10-14_16:03:06.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Zougman
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 03:50:58	ID requested
⏵ 1	2019-10-14 16:03:07	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: SiTrap, renal carcinoma

submitter keyword: SiTrap, renal carcinoma

Contact List

Alexandre Zougman
contact affiliation	University of Leeds
contact email	a.zougman@leeds.ac.uk
lab head
Alexandre Zougman
contact affiliation	University of Leeds
contact email	a.zougman@leeds.ac.uk
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084405/