PXD015673

PXD015673 is an original dataset announced via ProteomeXchange.

Title	Regulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα
Description	Progenitor cells at the basal layer of skin epidermis play an essential role in maintaining tissue homeostasis and enhancing wound repair in skin. The proliferation, differentiation, and cell death of epidermal progenitor cells have to be delicately regulated, as deregulation of this process can lead to many skin diseases, including skin cancers. However, the underlying molecular mechanisms involved in skin homeostasis remain poorly defined. In this study, with quantitative proteomics approach, we identified an important interaction between KDF1 (Keratinocyte Differentiation Factor 1) and IKKα (IκB kinase α) in differentiating skin keratinocytes. Ablation of either KDF1 or IKKα in mice leads to similar but striking abnormalities in skin development, particularly in skin epidermal differentiation. With biochemical and mouse genetics approach, we further demonstrate that the interaction of IKKα and KDF1 is essential for epidermal differentiation. To probe deeper into the mechanisms, we find that KDF1 associates with a deubiquitinating protease, USP7 (Ubiquitin Specific Peptidase 7), and KDF1 can regulate skin differentiation through deubiquitination and stabilization of IKKα. Taken together, our study unravels an important molecular mechanism underlying skin tissue homeostasis and epidermal differentiation.
HostingRepository	PRIDE
AnnounceDate	2020-05-13
AnnouncementXML	Submission_2020-05-12_22:22:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015673
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Phospho; Label:2H(4); Formyl; Dehydrated; Oxidation; Label:13C(6); Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 01:15:15	ID requested
⏵ 1	2020-05-12 22:22:34	announced

Li Y, Tang L, Yue J, Gou X, Lin A, Weatherbee SD, Wu X, . EMBO Rep, 21(5):e48566(2020) [pubmed]

submitter keyword: skin, epidermal differentiation, KDF1, IKKα, deubiquitination, SILAC, QE-MS

Xiaoyang Wu
contact affiliation	Ben May Department for Cancer Research, the University of Chicago, Chicago, IL 60637, USA.
contact email	xiaoyangwu@uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015673

PRIDE project URI

• PRIDE
  1. PXD015673
     1. Label: PRIDE project
     2. Name: Regulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα