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PXD015673

PXD015673 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRegulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα
DescriptionProgenitor cells at the basal layer of skin epidermis play an essential role in maintaining tissue homeostasis and enhancing wound repair in skin. The proliferation, differentiation, and cell death of epidermal progenitor cells have to be delicately regulated, as deregulation of this process can lead to many skin diseases, including skin cancers. However, the underlying molecular mechanisms involved in skin homeostasis remain poorly defined. In this study, with quantitative proteomics approach, we identified an important interaction between KDF1 (Keratinocyte Differentiation Factor 1) and IKKα (IκB kinase α) in differentiating skin keratinocytes. Ablation of either KDF1 or IKKα in mice leads to similar but striking abnormalities in skin development, particularly in skin epidermal differentiation. With biochemical and mouse genetics approach, we further demonstrate that the interaction of IKKα and KDF1 is essential for epidermal differentiation. To probe deeper into the mechanisms, we find that KDF1 associates with a deubiquitinating protease, USP7 (Ubiquitin Specific Peptidase 7), and KDF1 can regulate skin differentiation through deubiquitination and stabilization of IKKα. Taken together, our study unravels an important molecular mechanism underlying skin tissue homeostasis and epidermal differentiation.
HostingRepositoryPRIDE
AnnounceDate2020-05-13
AnnouncementXMLSubmission_2020-05-12_22:22:33.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD015673
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDonald Wolfgeher
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListAmmonia-loss; Phospho; Label:2H(4); Formyl; Dehydrated; Oxidation; Label:13C(6); Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-02 01:15:15ID requested
12020-05-12 22:22:34announced
Publication List
Li Y, Tang L, Yue J, Gou X, Lin A, Weatherbee SD, Wu X, . EMBO Rep, 21(5):e48566(2020) [pubmed]
Keyword List
submitter keyword: skin, epidermal differentiation, KDF1, IKKα, deubiquitination, SILAC, QE-MS
Contact List
Xiaoyang Wu
contact affiliationBen May Department for Cancer Research, the University of Chicago, Chicago, IL 60637, USA.
contact emailxiaoyangwu@uchicago.edu
lab head
Donald Wolfgeher
contact affiliationUniversity of Chicago
contact emaildonw@uchicago.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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