PXD015654

PXD015654 is an original dataset announced via ProteomeXchange.

Title	Yeast ubiquitin ligase LC-MS/MS
Description	Ubiquitin ligases (E3s) serves as a key regulator for ubiquitylation-mediated pathway. The identification of the corresponding relationship between E3 and its substrates is challenging but required for understanding the regulatory network of ubiquitylation. The low abundance of ubiquitinated conjugates and high redundancy of E3s-substrates regulation made screening pretty hard. Herein, we combined SILAC-based quantitative proteomics with two contrary genetic methods (overexpression and knockout) in theory for E3 (Hrt3, F-box subunit of SCF complex) substrates screening.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:09:10.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yihao Wang
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-01 02:13:55	ID requested
1	2019-12-06 01:55:58	announced
⏵ 2	2024-10-22 04:09:12	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00673;

Lan Q, Wang Y, Sun Z, Li Y, Zhang C, Chang L, Gao Y, Wu J, Wang F, Xu P, Quantitative Proteomics Combined with Two Genetic Strategies for Screening Substrates of Ubiquitin Ligase Hrt3. J Proteome Res, 19(1):493-502(2020) [pubmed]

submitter keyword: Hrt3;ubiquitinated substrates;

Ping Xu
contact affiliation	Beijing Proteome Research Center, National Center for Protein Sciences (Beijing) Beijing Institute of Lifeomics， Beijing 102206 P.R. China.
contact email	xuping_bprc@126.com
lab head
Yihao Wang
contact affiliation	NCPSB
contact email	hao_god@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015654

PRIDE project URI

• PRIDE
  1. PXD015654
     1. Label: PRIDE project
     2. Name: Yeast ubiquitin ligase LC-MS/MS