PXD015653

PXD015653 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis of symmetric arginine dimethylation in colorectal cancer tissues from patients using immuniaffinity enrichment followed by LC-MS/MS analysis
Description	Post-translational arginine methylation is responsible for regulation of a variety of biological processes. The protein arginine methyltransferase 5 (PRMT5) is the major enzyme responsible for generating monomethyl- and symmetric dimethyl arginine (MMA and SDMA, respectively) in proteins. PRMT5 is essential for viability and normal development, and overexpressed in a wide variety of cancer types. In the present study, we found that the levels of PRMT5 and symmetric dimethylation in proteins from colorectal cancer (CRC) tissues are more highly maintained relative to adjacent normal tissues from patients. Using immunoaffinity enrichment of methylated peptides combined with high resolution mass spectrometry, a total of 147 SDMA sites from 94 proteins were identified. Most abundant methylated proteins identified from normal and CRC tissues are RNA processing proteins, transcriptional and translational regulators. Furthermore, when quantitative analysis of the two tissue types of samples was carried out, 77 SDMA sites exhibited statistically significant changes (>2.0-fold, p-value <0.05) between normal and CRC tissues. We confirmed KSRP, G3BP2 and TRIP6 which are more highly maintained in cancer tissues relative to adjacent normal tissues from same patients as well as the expression levels of the proteins. This study is the first comprehensive analysis of symmetric arginine dimethylation using clinical samples and suggests that the modification can be used in clinical application.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:06:30.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yae Eun Park
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-01 02:03:29	ID requested
1	2020-06-01 04:51:36	announced
⏵ 2	2024-10-22 05:06:31	announced	2024-10-22: Updated project metadata.

Lim Y, Park YE, Ha S, Lee JE, Kim HC, A Comprehensive Analysis of Symmetric Arginine Dimethylation in Colorectal Cancer Tissues Using Immunoaffinity Enrichment and Mass Spectrometry. Proteomics, 20(15-16):e1900367(2020) [pubmed]

10.1002/pmic.201900367;

submitter keyword: PRMT5, Immunoaffinity enrichment,Colorectal cancer, Mass spectrometry, Symmetric arginine methylation

Ji Eun Lee
contact affiliation	Center for Teragnosis, biomedical research institute, Korea Institute of Science and Technology, Seoul, Korea
contact email	jelee9137@kist.re.kr
lab head
Yae Eun Park
contact affiliation	KIST
contact email	yaeeun1107@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015653
     1. Label: PRIDE project
     2. Name: Comprehensive analysis of symmetric arginine dimethylation in colorectal cancer tissues from patients using immuniaffinity enrichment followed by LC-MS/MS analysis