PXD015625

PXD015625 is an original dataset announced via ProteomeXchange.

Title	Freshwater benthic cyanobacterial biofilm LC-MSMS
Description	In order to understand how Phormidium mats establish, and the role of associated taxa in their development, we collected biofilms over a 19-day growth period during a nitrate-induced bloom event in the Wai-iti River for proteogenomics analysis. At the onset of a late summer bloom, cobbles from the Wai-iti River (Nelson, New Zealand) were removed, cleared of incipient growth with sterile sponges, and placed back into the river. Clearing was gentle as seeding from the pre-existing rock surface is important for bloom establishment [24]. Five pre-cleared cobbles were collected at each of 3 time points to capture the first 3, 6 and 9 days of growth (Table S1). Additional cobbles that contemporaneously developed biofilms were collected at days 12 and 19.
HostingRepository	PRIDE
AnnounceDate	2020-05-19
AnnouncementXML	Submission_2020-08-11_05:33:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hwee Sze Tee
SpeciesList	scientific name: uncultured Phormidium sp.; NCBI TaxID: 259949;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-27 08:27:07	ID requested
1	2020-05-18 21:43:31	announced
⏵ 2	2020-08-11 05:33:55	announced	2020-08-11: Updated publication reference for PubMed record(s): 32424245.

Tee HS, Waite D, Payne L, Middleditch M, Wood S, Handley KM, Tools for successful proliferation: diverse strategies of nutrient acquisition by a benthic cyanobacterium. ISME J, 14(8):2164-2178(2020) [pubmed]

curator keyword: Biological

submitter keyword: Microcoleus, LC-MSMS

Kim Handley
contact affiliation	School of Biological Sciences, University of Auckland
contact email	kim.handley@auckland.ac.nz
lab head
Hwee Sze Tee
contact affiliation	University of Auckland
contact email	htee663@aucklanduni.ac.nz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015625

PRIDE project URI

• PRIDE
  1. PXD015625
     1. Label: PRIDE project
     2. Name: Freshwater benthic cyanobacterial biofilm LC-MSMS