PXD015623

PXD015623 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of iron limitation in Klebsiella pneumoniae
Description	Nutrient adaptation is key in limiting environments for the promotion of microbial growth and survival. In microbial systems, iron is an essential component for many cellular processes and bioavailability varies greatly among different conditions. In the bacterium, Klebsiella pneumoniae, the impact of iron limitation is known to alter transcriptional expression of iron-acquisition pathways and influences the secretion of iron-binding siderophores; however, a comprehensive view of iron limitation at the protein-level remains to be defined. Here, we apply a mass spectrometry-based quantitative proteomics strategy to profile the global impact of iron limitation on the cellular proteome and extracellular environment (secretome) of K. pneumoniae. Our data defines the impact of iron on proteins involved in transcriptional regulation and emphasizes the modulation of a vast array of proteins associated with iron acquisition, transport, and binding. We also identify proteins in the extracellular environment associated with conventional and nonconventional modes of secretion, as well as vesicle release. In particular, we demonstrate a new role for Lon protease in promoting iron homeostasis outside of the cell. Our characterization of Lon protease in K. pneumoniae validates roles in bacterial growth, cell division, iron utilization, and virulence. Moreover, our results uncover novel degradation candidates of Lon protease. Overall, we provide evidence of novel connections between Lon and iron in a bacterial system and define a unique role for Lon protease in the extracellular environment during nutrient limitation.
HostingRepository	PRIDE
AnnounceDate	2020-03-09
AnnouncementXML	Submission_2020-06-12_02:35:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: 573;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-27 08:21:34	ID requested
1	2020-03-09 00:56:42	announced
⏵ 2	2020-06-12 02:35:26	announced	2020-06-12: Updated publication reference for PubMed record(s): 32390954.

Muselius B, Sukumaran A, Yeung J, Geddes-McAlister J, Defines New Roles for Lon Protease in Homeostasis and Degradation by Quantitative Proteomics. Front Microbiol, 11():546(2020) [pubmed]

submitter keyword: Quantitative proteomics, iron limitation, Klebsiella pneumoniae, secretome, Lon protease

Jennifer Geddes-McAlister
contact affiliation	Assistant Professor, Molecular and Cellular Biology Departmetn, University of Guelph
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015623
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of iron limitation in Klebsiella pneumoniae