PXD015620

PXD015620 is an original dataset announced via ProteomeXchange.

Title	Aptamer Affinity Mass Spectrometry
Description	Antibody-based affinity purification of macromolecular complexes has revolutionized the study of protein-protein interactions. Here, we present AptA-MS (Aptamer Affinity – Mass Spectrometry), a robust strategy using a specific, high-affinity RNA aptamer against GFP to identify novel interactors of a GFP-tagged protein using high resolution MS. AptA-MS offers a high signal-to-noise ratio due to the absence of immunoprecipitation-derived contaminants and allows the identification of post-translational modifications without the need for modification-specific enrichments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:27.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angela Kruse
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-27 06:12:22	ID requested
1	2020-06-16 23:01:30	announced
2	2020-07-07 22:50:29	announced	2020-07-08: Updated publication reference for PubMed record(s): 32609809.
⏵ 3	2024-10-22 05:07:28	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkaa542;

Ray J, Kruse A, Ozer A, Kajitani T, Johnson R, MacCoss M, Heck M, Lis JT, RNA aptamer capture of macromolecular complexes for mass spectrometry analysis. Nucleic Acids Res, 48(15):e90(2020) [pubmed]

submitter keyword: HCT116, Aptamer,RNA Aptamer, Heat Shock Factor, S2

Michelle Cilia Heck
contact affiliation	Department of Plant Pathology and Plant Microbe Biology, Cornell University, United States of America
contact email	mlc68@cornell.edu
lab head
Angela Kruse
contact affiliation	Vanderbilt University
contact email	ark253@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD015620

PRIDE project URI

• PRIDE
  1. PXD015620
     1. Label: PRIDE project
     2. Name: Aptamer Affinity Mass Spectrometry