PXD015617

PXD015617 is an original dataset announced via ProteomeXchange.

Title	Environmental conditions steer phenotypic switching in AHPND-causing Vibrio parahaemolyticus, affecting PirABVP toxin production
Description	Bacteria in nature are widely exposed to differential fluid shears which are often a trigger for phenotypic switches. The latter mediates transcriptional and translation remodeling of cellular metabolism impacting among others virulence, antimicrobial resistance and stress resistance. In this study, we evaluated the role of fluid shear on phenotypic switch in an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus M0904 strain under both in vitro and in vivo conditions. The results showed that V. parahaemolyticus M0904 grown at lower shaking speed (110 min-1 constant agitation, M0904/110), causing low fluid shear, develop cellular aggregates or floccules. These cells increased levan production (as verified by concanavalin binding) and developed differentially stained colonies on Congo Red agar plates and resistance to antibiotics. In addition, the phenotypic switch causes a major shift in the protein secretome. At 120 min-1 (M0904/120), PirA/B toxins are mainly produced, while at 110 min-1 PirA/B toxin production is stopped and an alkaline phosphatase PhoX becomes the dominant protein in the protein secretome. These observations are matched with a very strong reduction in virulence of M0904/110 towards two crustacean larvae, namely Artemia and Macrobrachium. Taken together, our study provides substantial evidence for the existence of two phenotypic forms in AHPND Vibrio parahaemolyticus strains displaying differential phenotypes that could be of interest in understanding the epidemiology of AHPND under aquaculture conditions. It might provide the basis for AHPND control by steering phenotypes.
HostingRepository	PRIDE
AnnounceDate	2020-02-05
AnnouncementXML	Submission_2020-05-18_05:28:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Delphi Van Haver
SpeciesList	scientific name: Vibrio parahaemolyticus; NCBI TaxID: 670;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-27 06:11:41	ID requested
1	2020-02-05 06:02:45	announced
⏵ 2	2020-05-18 05:28:52	announced	2020-05-18: Updated publication reference for PubMed record(s): 31867836.

Kumar V, Roy S, Baruah K, Van Haver D, Impens F, Bossier P, toxins production. Environ Microbiol, 22(10):4212-4230(2020) [pubmed]

submitter keyword: Vibrio parahaemolyticus, fluid shear, phenotype switching, biological features, virulence, antimicrobial resistance

Peter Bossier
contact affiliation	Lab of Aquaculture & Artemia Reference Center, Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
contact email	Peter.Bossier@ugent.be
lab head
Delphi Van Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015617
     1. Label: PRIDE project
     2. Name: Environmental conditions steer phenotypic switching in AHPND-causing Vibrio parahaemolyticus, affecting PirABVP toxin production