PXD015611

PXD015611 is an original dataset announced via ProteomeXchange.

Title	Quantitative Rps2 BioID to monitor Asc1-dependent rearrangement at the head region of the 40S ribosome
Description	In a previous study we applied a quantitative proximity-labeling technique called Biotin Identification (BioID, Roux et al., 2012) to analyze the microenvironment of the Gβ-like scaffold protein Asc1 (Opitz et al., 2017). The WD40-repeat protein Asc1 binds to the head region of the small 40S ribosomal (hr40S) subunit. In this study, we analyzed the hr40S from the perspective of Rps2, a ribosomal neighbor of Asc1. We intended to confirm proteins of the Asc1 proxiOME at the hr40S and to observe changes when Asc1 was absent. References: Roux K.J., Kim D.I., Raida M., Burke B. 2012. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J Cell Biol 196:801-810 Opitz N., Schmitt K., Hofer-Pretz V., Neumann B., Krebber H., Braus G.H., Valerius O. 2017. Capturing the Asc1p/RACK1 microenvironment at the head region of the 40S ribosome with quantitative BioID in yeast. Mol Cell Proteomics 16:2199-2218
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:09:58.233.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-26 23:56:08	ID requested
1	2019-11-07 04:56:30	announced
2	2019-11-08 00:37:59	announced	2019-11-08: Updated publication reference for PubMed record(s): 31689955.
3	2019-11-27 06:52:55	announced	2019-11-27: Updated project metadata.
⏵ 4	2024-10-22 04:10:06	announced	2024-10-22: Updated project metadata.

10.3390/cells8111384;

Schmitt K, Valerius O, yRACK1/Asc1 proxiOMICs-Towards Illuminating Ships Passing in the Night. Cells, 8(11):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Asc1, Bre5, Sro9, BioID, Def1, Stm1, Rps2, SILAC, ribosome, Rps3,translation, Ubp3,Saccharomyces cerevisiae, Vps30

Oliver Valerius
contact affiliation	Georg-August-University Göttingen, Department of Molecular Microbiology and Genetics, Institute of Microbiology and Genetics
contact email	ovaleri@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015611

PRIDE project URI

• PRIDE
  1. PXD015611
     1. Label: PRIDE project
     2. Name: Quantitative Rps2 BioID to monitor Asc1-dependent rearrangement at the head region of the 40S ribosome