PXD015599

PXD015599 is an original dataset announced via ProteomeXchange.

Title	Identification of Bona Fide Targets of AMPylating Fic-enzymes by Substrate Mediated Covalent Capture
Description	Many pathogenic bacteria utilize posttranslational modifications to manipulate central components of the host cell functions. Often, the released enzymes belong to the large Fic-family, causing target modification with nucleotide monophosphates. Due to the lack of a generic method for the identification of the Fic-family’s cellular targets, we have established a new approach by combining synthetic thiol-reactive nucleotide derivatives (TReNDs) with recombinantly produced Fic-enzymes containing strategically placed cysteines in their active sites, thereby producing reactive binary FicTReND probes for covalent substrate capture. The binary probes successfully capture their targets from complex lysates and permit their identification by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2020-07-09
AnnouncementXML	Submission_2020-07-09_02:17:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christoph Krisp
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-26 01:55:50	ID requested
⏵ 1	2020-07-09 02:17:41	announced

Gulen B, Rosselin M, Fauser J, Albers MF, Pett C, Krisp C, Pogenberg V, Schl, ü, ter H, Hedberg C, Itzen A, Identification of targets of AMPylating Fic enzymes by co-substrate-mediated covalent capture. Nat Chem, 12(8):732-739(2020) [pubmed]

curator keyword: Biological

submitter keyword: Post-translational modifications, AMPylation, Fic enzymes, Host-pathogen interactions

Azmelt tyen
contact affiliation	University Medical Center Hamburg Eppendorf Center for Experimental Medicine, Institute of Biochemisry and Signaltransduction Hamburg Germany
contact email	a.itzen@uke.de
lab head
Christoph Krisp
contact affiliation	University Medical Center Hamburg-Eppendorf Institute of Clinical Chemistry and Laboratory Medicine Mass Spectrometric Proteomics
contact email	c.krisp@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015599

PRIDE project URI

• PRIDE
  1. PXD015599
     1. Label: PRIDE project
     2. Name: Identification of Bona Fide Targets of AMPylating Fic-enzymes by Substrate Mediated Covalent Capture