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PXD015584

PXD015584 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative Analysis of Global Protein Stability Rates in Tissues
DescriptionWe have developed QUAD (Quantification of Azidohomoalanine Degradation), a technique to quantitate global protein degradation using mass spectrometry. Azidohomoalanine (AHA) is pulsed into mouse tissues through their diet. The mice are then returned to a normal diet and the decrease of AHA abundance can be quantitated in the proteome. QUAD analysis reveals that protein stability varied within tissues, but discernible trends in the data suggest that cellular environment is a major factor dictating stability. Within a tissue, different organelles, post-translation modifications, and protein functions were enriched with different stability patterns. Surprisingly, subunits of the TRIC molecular chaperonin possessed markedly distinct stability trajectories in the brain. Further investigation revealed that these subunits also possessed different subcellular localization indicating a potential non-canonical chaperonin. Finally, QUAD analysis demonstrated that protein stability is enhanced with age in the brain but not in the liver. Overall, QUAD allows the first global quantitation of protein stability rates in tissues, which may lead to new insights and hypotheses in basic and translational research.
HostingRepositoryMassIVE
AnnounceDate2020-09-08
AnnouncementXMLSubmission_2020-09-08_13:57:05.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel McClatchy
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListMet->Aha
InstrumentLTQ Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-25 09:32:44ID requested
12020-09-08 13:57:05announced
Publication List
no publication
Keyword List
submitter keyword: Azidohomoalanine, PALM, AHA, degradation, QUAD
Contact List
John R. Yates III
contact affiliationThe Scripps Research Institute
contact emailjyates@scripps.edu
lab head
Daniel McClatchy
contact affiliationTSRI
contact emaildmcclat@scripps.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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