PXD015577

PXD015577 is an original dataset announced via ProteomeXchange.

Title	Calsequestrins make the difference in adult zebrafish brain
Description	Aim of the study was to examine the expression of calsequestrin isoforms in zebrafish adult brain. By quantitative PCR we found three RNA transcripts coding for Casq1a, Casq1b and Casq2. In brain homogenate, two protein isoforms were detected by western blotting and mass spectrometry. Whole brain fractionation experiments revealed that Casq2 isoform was enriched in a heavy fraction containing microsomes and synaptic membranes, while Casq1a was recovered also in a light cytosolic-enriched fraction. By immunofluorescence, we found that calsequestrins show different cellular localization and we confirmed heterogeneous cell expression of Casq1a and Casq2 mRNA by in situ hybridization. Both isoforms were expressed in cerebellum, and cerebellum-like structures of the brain, Casq2 more concentrated in Purkinje cells, and Casq1 equally distributed in granular and Purkinje cells. At sub cellular level, in Purkinje cells, Casq2 was localized in cell bodies, dendrites and axons, while Casq1 was restricted to cell bodies and axons. Data are discussed in relation to the heterogeneous cellular and subcellular distribution of other Calcium store markers of cerebellum and are evaluated with respect to the relevance of calsequestrins in neuron-specific functional activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:44.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Murgia
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-25 02:30:24	ID requested
1	2020-05-26 15:35:05	announced
⏵ 2	2024-10-22 05:05:48	announced	2024-10-22: Updated project metadata.

Furlan S, Campione M, Murgia M, Mosole S, Argenton F, Volpe P, Nori A, Calsequestrins New Calcium Store Markers of Adult Zebrafish Cerebellum and Optic Tectum. Front Neuroanat, 14():15(2020) [pubmed]

10.3389/fnana.2020.00015;

curator keyword: Biological

submitter keyword: Calsequestrin, Danio rerio, cerebellum, adult

Alessandra Nori
contact affiliation	Department of Biomedical Sciences, University of Padova, Italy
contact email	alessandra.nori@unipd.it
lab head
Marta Murgia
contact affiliation	MPI Biochemistry
contact email	mmurgia@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015577

PRIDE project URI

• PRIDE
  1. PXD015577
     1. Label: PRIDE project
     2. Name: Calsequestrins make the difference in adult zebrafish brain