PXD015574

PXD015574 is an original dataset announced via ProteomeXchange.

Title	Regulated local synthesis of ribosomal proteins maintains ribosome function in axons
Description	Ribosome assembly occurs mainly in the nucleolus yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a novel sequence motif, CUIC, that forms an RNA-loop structure in the region immediately upstream of the initiation codon. Using imaging and subcellular proteomics techniques, we show that RPs synthesized in axons join axonal ribosomes in a nucleolus-independent fashion. Inhibition of axonal CUIC-regulated RP translation causes a significant decline in local translation activity and markedly reduces axon branching in the brain, revealing the physiological relevance of axonal RP synthesis in vivo. These results suggest that axonal translation supplies cytoplasmic RPs to maintain/modify local ribosomal function far from the nucleolus in neurons.
HostingRepository	PRIDE
AnnounceDate	2019-12-09
AnnouncementXML	Submission_2019-12-09_04:09:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toshiaki Shigeoka
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-25 02:01:32	ID requested
⏵ 1	2019-12-09 04:09:43	announced

Dataset with its publication pending

submitter keyword: ribosomes, Sucrose density gradient fractionation, axon

Christine E Holt
contact affiliation	Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
contact email	ceh33@cam.ac.uk
lab head
Toshiaki Shigeoka
contact affiliation	University of Cambridge
contact email	ts561@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015574
     1. Label: PRIDE project
     2. Name: Regulated local synthesis of ribosomal proteins maintains ribosome function in axons