PXD015558

PXD015558 is an original dataset announced via ProteomeXchange.

Title	An optimized apoplastic fluid extraction method for the simultaneous characterization of the proteome and metabolome of grapevine leaves.
Description	The study of different cellular compartments also allows a broader conception of cell dynamics. Plant apoplast is the cellular compartment external to the plasma membrane including the cell wall. Despite our knowledge on apoplast involvement on several processes from cell growth to biotic and abiotic stress response, its dynamics is yet still poorly studied due to the need for adaptation of the extraction process according to the plant system and consequently, the difficulty to perform a comprehensive profiling of proteins, metabolites and lipids within a single experiment. Moreover, when looking at woody plants as grapevine, more challenges are raised leading to only few studies published so far focusing on apoplast characterization. Being grapevine one of the most important fruit crops worldwide, a wider characterization of this compartment may unveil the importance of grapevine’s secretome. Here, we describe an optimized vacuum-infiltration-centrifugation method for the simultaneous extraction of apoplastic proteins and metabolites from the grapevine leaf on a single experiment, compatible with high-throughput mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:11:26.834.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015558
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationList	Trp->Kynurenin; Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-24 02:17:06	ID requested
1	2021-02-16 23:46:25	announced
⏵ 2	2023-11-14 07:11:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD015558;

submitter keyword: proteins, extracellular space, mass spectrometry, carbohydrates, lipids,Vitis vinifera, comprehensive analysis

Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-Green Tech Integrative biology platform Belvaux, LUXEMBOURG
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-BEAP-HAU platform
contact email	celine.leclercq@list.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD015558

PRIDE project URI

• PRIDE
  1. PXD015558
     1. Label: PRIDE project
     2. Name: An optimized apoplastic fluid extraction method for the simultaneous characterization of the proteome and metabolome of grapevine leaves.