PXD015551

PXD015551 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA)
Description	Somatic embryos are very much similar to zygotic counterparts in many morphological aspects and the somatic embryos are derived from somatic cells by undergoing various metabolic regulations. The somatic embryos have been used in artificial seed technology, genetic engineering and germplasm conservation. Though somatic embryo development is an important topic in growth and developmental studies, the molecular mechanism underlying the developmental process remains unclear. Therefore, understanding the molecular basis behind somatic embryo development can provide insight on the signaling pathways integrating this process. Proteomic analysis of somatic embryo development in cv. Grand Naine (AAA) was carried out to identify the differentially accumulated protein using two dimensional gel electrophoresis coupled with mass spectrometry. In total, 25 protein spots were differentially accumulated in different developmental stages of somatic embryos. Among them, three proteins were uniquely present in 30 days globular stage somatic embryos and six proteins were uniquely present in 60 days matured somatic embryo. Functional annotation of identified spots showed that major proteins are involved in growth and developmental process (17 %) followed by defense response (12%) and signal transportation events (12 %). In early stage, cell division and growth related proteins were involved in the induction of somatic embryos whereas in late developmental stage, cell wall modification proteins along with stress related proteins like played a defense role against dehydration and osmotic stress and resulted in maturation of somatic embryo. Alongside some identified stage specific proteins are valuable indicators and have been used as genetic markers.
HostingRepository	PRIDE
AnnounceDate	2020-03-16
AnnouncementXML	Submission_2020-06-12_02:47:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kumaravel Marimuthu
SpeciesList	scientific name: Musa acuminata AAA Group; NCBI TaxID: 214697;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-23 07:24:08	ID requested
1	2020-03-16 02:21:33	announced
⏵ 2	2020-06-12 02:47:57	announced	2020-06-12: Updated publication reference for PubMed record(s): 32161309.

Kumaravel M, Uma S, Backiyarani S, Saraswathi MS, Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA). Sci Rep, 10(1):4501(2020) [pubmed]

submitter keyword: Banana, proteomics, somatic embryos, development

UMA SUBBARAYA
contact affiliation	Director, National Research Centre for Banana, Trichy, Tamil Nadu, India
contact email	umabinit@yahoo.co.in
lab head
Kumaravel Marimuthu
contact affiliation	Senior Research Fellow Crop Improvement Division National Research Centre for Banana, Tiruchirappalli, Tamil Nadu, India
contact email	velu_bai@yahoo.co.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015551
     1. Label: PRIDE project
     2. Name: Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA)