PXD015547

PXD015547 is an original dataset announced via ProteomeXchange.

Title	Comprehensive map of the Artemisia annua proteome and quantification of differential protein expression in chemotypes producing high vs low content of artemisinin
Description	Artemisia annua is well known for biosynthesizing artemisinin, which is the primary therapeutic approach against malaria. It was reported that treatment with leaf of A. annua showed better effect and less tendency of developing drug resistance than purified artemisinin, suggesting other components in A. annua may contribute to the therapeutic efficacy. Here, we conducted a global proteomic profiling of A. annua with identification of a total of 13,403 proteins based on the genome sequence annotation database. Furthermore, we generated a spectral library to perform quantitative proteomic analysis using data independent acquisition mass spectrometry (DIA-MS). Specifically, we comprehensively quantified and compared proteins between two chemotypes that produce high (HAP) and low (LAP) artemisinin content, respectively. 182 proteins were identified with abundance significantly different between these two chemotypes. Overall, our current study globally identified the proteome of A. annua and quantitatively compared the targeted sub-proteomes between the two cultivars of HAP and LAP, providing systematic information on metabolic pathways of A. annua and facilitating identification of good chemotypes for producing anti-malaria compounds.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-04-26_22:51:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MINGHUI CHEN
SpeciesList	scientific name: Artemisia annua (Sweet wormwood); NCBI TaxID: 35608;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-23 03:02:21	ID requested
⏵ 1	2020-04-26 22:51:48	announced

Chen M, Yan T, Ji L, Dong Y, Sidoli S, Yuan Z, Cai C, Chen J, Tang Y, Shen Q, Pan Q, Fu X, Ku X, Liao L, Garcia BA, Yan W, Tang K, Comprehensive Map of the Artemisia annua Proteome and Quantification of Differential Protein Expression in Chemotypes Producing High versus Low Content of Artemisinin. Proteomics, 20(10):e1900310(2020) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

curator keyword: Biomedical

submitter keyword: Artemisia annua, proteome, metabolism, Data dependent acquisition, Data independent acquisition, mass spectrometry

Kexuan Tang
contact affiliation	Joint International Research Laboratory of Metabolic & Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China
contact email	kxtang@sjtu.edu.cn
lab head
MINGHUI CHEN
contact affiliation	postdoc
contact email	chmhui@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015547
     1. Label: PRIDE project
     2. Name: Comprehensive map of the Artemisia annua proteome and quantification of differential protein expression in chemotypes producing high vs low content of artemisinin