PXD015543

PXD015543 is an original dataset announced via ProteomeXchange.

Title	Intestinal response to dietary manganese depletion in Drosophila
Description	Manganese is considered essential for animal growth. Manganese ions serve as cofactors to three mitochondrial enzymes: superoxide dismutase (Sod2), arginase and glutamine synthase. In Drosophila melanogaster, manganese has also been implicated in the formation of ceramide phosphoethanolamine, the insect’s sphingomyelin analogue, a structural component of membranes. Manganese overload leads to neurodegeneration and toxicity in both humans and Drosophila. Here, we describe a Drosophila model of manganese deficiency. Due to the lack of manganese-specific chelators, we used chemically defined media to grow the flies and deplete them of the metal. Dietary manganese depletion reduces Sod2 activity. We then examined gene and protein expression changes in the intestines of manganese depleted flies. We found adaptive responses to the lack of the known manganese-dependent enzymatic activities and alterations in genes/enzymes of carbohydrate metabolism and glycosylations, similar to earlier reports of manganese deficiency in vertebrate animals.
HostingRepository	PRIDE
AnnounceDate	2020-02-24
AnnouncementXML	Submission_2020-02-24_02:37:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johana Vásquez
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	carbamoylated residue; phosphorylated residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-23 01:32:58	ID requested
⏵ 1	2020-02-24 02:37:20	announced

V, á, squez-Procopio J, Osorio B, Cort, é, s-Mart, í, nez L, Hern, á, ndez-Hern, á, ndez F, Medina-Contreras O, R, í, os-Castro E, Comjean A, Li F, Hu Y, Mohr S, Perrimon N, Missirlis F, Intestinal response to dietary manganese depletion in Drosophila. Metallomics, 12(2):218-240(2020) [pubmed]

submitter keyword: Superoxide dismutase (Sod2), white + (w+)

Fanis Missirlis
contact affiliation	Lab head
contact email	fanis@fisio.cinvestav.mx
lab head
Johana Vásquez
contact affiliation	Cinvestav
contact email	29yoana29@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD015543

PRIDE project URI

• PRIDE
  1. PXD015543
     1. Label: PRIDE project
     2. Name: Intestinal response to dietary manganese depletion in Drosophila