PXD015538 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | WblC- and tetracycline-dependent shifts in the composition of Streptomyces coelicolor ribosomal proteome |
Description | WblC, also known as WhiB7, is a widely conserved WhiB-like transcription factor in actinomycetes that activates transcription of many targets upon antibiotic challenge to bring about intrinsic resistance to a wide range of translation-targeting antibiotics. As we found that WblC controls many genes involved in translation and that WblC promotes translation rate upon antibiotic stress in the model actinomycetes Streptomyces coelicolor, we speculated that WblC might alter the protein composition of ribosome during antibiotic stress. To test this, we prepared 70S ribosome fraction from wild-type S. coelicolor cells untreated or treated with tetracycline and ΔwblC mutant treated with tetracycline, and then compared the protein compositions of each 70S samples by mass spectrometric quantification. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:05:50.226.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ju-Hyung Lee |
SpeciesList | scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226; |
ModificationList | L-methionine sulfoxide; alpha-amino acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-23 01:32:10 | ID requested | |
1 | 2020-05-26 15:43:32 | announced | |
⏵ 2 | 2024-10-22 05:05:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Lee JH, Yoo JS, Kim Y, Kim JS, Lee EJ, Roe JH, The WblC/WhiB7 Transcription Factor Controls Intrinsic Resistance to Translation-Targeting Antibiotics by Altering Ribosome Composition. mBio, 11(2):(2020) [pubmed] |
10.1128/mbio.00625-20; |
Keyword List
submitter keyword: Streptomyces coelicolor, ribosome, WhiB7,WblC, antibiotic stress |
Contact List
Jung-Hye Roe |
contact affiliation | School of Biological Sciences, Seoul National University, Seoul, 08826, South Korea |
contact email | jhroe@snu.ac.kr |
lab head | |
Ju-Hyung Lee |
contact affiliation | Seoul National University |
contact email | s10jhl@snu.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015538
- Label: PRIDE project
- Name: WblC- and tetracycline-dependent shifts in the composition of Streptomyces coelicolor ribosomal proteome