⮝ Full datasets listing

PXD015538

PXD015538 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleWblC- and tetracycline-dependent shifts in the composition of Streptomyces coelicolor ribosomal proteome
DescriptionWblC, also known as WhiB7, is a widely conserved WhiB-like transcription factor in actinomycetes that activates transcription of many targets upon antibiotic challenge to bring about intrinsic resistance to a wide range of translation-targeting antibiotics. As we found that WblC controls many genes involved in translation and that WblC promotes translation rate upon antibiotic stress in the model actinomycetes Streptomyces coelicolor, we speculated that WblC might alter the protein composition of ribosome during antibiotic stress. To test this, we prepared 70S ribosome fraction from wild-type S. coelicolor cells untreated or treated with tetracycline and ΔwblC mutant treated with tetracycline, and then compared the protein compositions of each 70S samples by mass spectrometric quantification.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:05:50.226.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJu-Hyung Lee
SpeciesList scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226;
ModificationListL-methionine sulfoxide; alpha-amino acetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-23 01:32:10ID requested
12020-05-26 15:43:32announced
22024-10-22 05:05:52announced2024-10-22: Updated project metadata.
Publication List
Lee JH, Yoo JS, Kim Y, Kim JS, Lee EJ, Roe JH, The WblC/WhiB7 Transcription Factor Controls Intrinsic Resistance to Translation-Targeting Antibiotics by Altering Ribosome Composition. mBio, 11(2):(2020) [pubmed]
10.1128/mbio.00625-20;
Keyword List
submitter keyword: Streptomyces coelicolor, ribosome, WhiB7,WblC, antibiotic stress
Contact List
Jung-Hye Roe
contact affiliationSchool of Biological Sciences, Seoul National University, Seoul, 08826, South Korea
contact emailjhroe@snu.ac.kr
lab head
Ju-Hyung Lee
contact affiliationSeoul National University
contact emails10jhl@snu.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015538
PRIDE project URI
Repository Record List
[ + ]