PXD015538

PXD015538 is an original dataset announced via ProteomeXchange.

Title	WblC- and tetracycline-dependent shifts in the composition of Streptomyces coelicolor ribosomal proteome
Description	WblC, also known as WhiB7, is a widely conserved WhiB-like transcription factor in actinomycetes that activates transcription of many targets upon antibiotic challenge to bring about intrinsic resistance to a wide range of translation-targeting antibiotics. As we found that WblC controls many genes involved in translation and that WblC promotes translation rate upon antibiotic stress in the model actinomycetes Streptomyces coelicolor, we speculated that WblC might alter the protein composition of ribosome during antibiotic stress. To test this, we prepared 70S ribosome fraction from wild-type S. coelicolor cells untreated or treated with tetracycline and ΔwblC mutant treated with tetracycline, and then compared the protein compositions of each 70S samples by mass spectrometric quantification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:50.226.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ju-Hyung Lee
SpeciesList	scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226;
ModificationList	L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-23 01:32:10	ID requested
1	2020-05-26 15:43:32	announced
⏵ 2	2024-10-22 05:05:52	announced	2024-10-22: Updated project metadata.

Lee JH, Yoo JS, Kim Y, Kim JS, Lee EJ, Roe JH, The WblC/WhiB7 Transcription Factor Controls Intrinsic Resistance to Translation-Targeting Antibiotics by Altering Ribosome Composition. mBio, 11(2):(2020) [pubmed]

10.1128/mbio.00625-20;

submitter keyword: Streptomyces coelicolor, ribosome, WhiB7,WblC, antibiotic stress

Jung-Hye Roe
contact affiliation	School of Biological Sciences, Seoul National University, Seoul, 08826, South Korea
contact email	jhroe@snu.ac.kr
lab head
Ju-Hyung Lee
contact affiliation	Seoul National University
contact email	s10jhl@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015538

PRIDE project URI

• PRIDE
  1. PXD015538
     1. Label: PRIDE project
     2. Name: WblC- and tetracycline-dependent shifts in the composition of Streptomyces coelicolor ribosomal proteome