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PXD015507

PXD015507 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of CTPS1 Filament Interacting Proteins_2019 dataset
DescriptionCTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Previously, we demonstrated that histidine (His) mediated methylation promotes the formation of CTPS filament to suppress the enzymatic activity and preserve CTPS protein under Gln deprivation, which promotes cancer cell growth after stress alleviation. However, it remains unclear where and how these enigmatic structures are assembled. Here we used APEX2-mediated in vivo proximity labeling to identify proteins involve in the formation of human CTPS filaments.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:01:18.216.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYu-Tsun Lin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-19 02:56:25ID requested
12020-03-24 03:41:45announced
22024-10-22 05:01:19announced2024-10-22: Updated project metadata.
Publication List
10.1242/jcs.240200;
Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM, SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci, 133(9):(2020) [pubmed]
Keyword List
submitter keyword: APEX2, TMT6 labeling,CTPS1, LC-MS/MS
Contact List
Jau-Song Yu
contact affiliationMolecular Medicine Research Center, Chang Gung University
contact emailyusong@mail.cgu.edu.tw
lab head
Yu-Tsun Lin
contact affiliationChang Gung University
contact emaildoreenlin1230@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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