PXD015507 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of CTPS1 Filament Interacting Proteins_2019 dataset |
Description | CTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Previously, we demonstrated that histidine (His) mediated methylation promotes the formation of CTPS filament to suppress the enzymatic activity and preserve CTPS protein under Gln deprivation, which promotes cancer cell growth after stress alleviation. However, it remains unclear where and how these enigmatic structures are assembled. Here we used APEX2-mediated in vivo proximity labeling to identify proteins involve in the formation of human CTPS filaments. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:01:18.216.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yu-Tsun Lin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-19 02:56:25 | ID requested | |
1 | 2020-03-24 03:41:45 | announced | |
⏵ 2 | 2024-10-22 05:01:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1242/jcs.240200; |
Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM, SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci, 133(9):(2020) [pubmed] |
Keyword List
submitter keyword: APEX2, TMT6 labeling,CTPS1, LC-MS/MS |
Contact List
Jau-Song Yu |
contact affiliation | Molecular Medicine Research Center, Chang Gung University |
contact email | yusong@mail.cgu.edu.tw |
lab head | |
Yu-Tsun Lin |
contact affiliation | Chang Gung University |
contact email | doreenlin1230@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015507
- Label: PRIDE project
- Name: Identification of CTPS1 Filament Interacting Proteins_2019 dataset