PXD015507

PXD015507 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of CTPS1 Filament Interacting Proteins_2019 dataset
Description	CTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Previously, we demonstrated that histidine (His) mediated methylation promotes the formation of CTPS filament to suppress the enzymatic activity and preserve CTPS protein under Gln deprivation, which promotes cancer cell growth after stress alleviation. However, it remains unclear where and how these enigmatic structures are assembled. Here we used APEX2-mediated in vivo proximity labeling to identify proteins involve in the formation of human CTPS filaments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:18.216.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu-Tsun Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-19 02:56:25	ID requested
1	2020-03-24 03:41:45	announced
⏵ 2	2024-10-22 05:01:19	announced	2024-10-22: Updated project metadata.

Publication List

10.1242/jcs.240200;
Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM, SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci, 133(9):(2020) [pubmed]

10.1242/jcs.240200;

Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM, SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci, 133(9):(2020) [pubmed]

Keyword List

submitter keyword: APEX2, TMT6 labeling,CTPS1, LC-MS/MS

submitter keyword: APEX2, TMT6 labeling,CTPS1, LC-MS/MS

Contact List

Jau-Song Yu
contact affiliation	Molecular Medicine Research Center, Chang Gung University
contact email	yusong@mail.cgu.edu.tw
lab head
Yu-Tsun Lin
contact affiliation	Chang Gung University
contact email	doreenlin1230@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD015507
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD015507

PRIDE project URI

Repository Record List

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