PXD015495

PXD015495 is an original dataset announced via ProteomeXchange.

Title	Profiling of retinaldehyde dehydrogenases in cancer cells
Description	Retinaldehyde dehydrogenases convert retinal into all-trans retinoic acid (ATRA), thereby regulating cell differentiation and cancer stem cell proliferation. Chemical tools and methods are valuable commodities to study aldehyde dehydrogenase (ALDH) activity in human cells. Here, we describe the design, synthesis and application of LEI-945, a first-in-class activity-based probe modeled on retinal that reports on individual ALDHs in cancer cells. Comparative chemical proteomics with LEI-945 explained the ability of various breast cancer cell lines to produce ATRA via retinaldehyde dehydrogenases isozymes, whereas the ALDEFLUORTM assay could not. Competitive chemical proteomics using LEI-945 revealed that the ALDH inhibitor NCT-505 inhibited both ALDH1A1 and ALDH1A3 in MDA-MB-468 breast cancer cells inducing a cell cycle arrest in the G1 phase as well as cell death through necrosis. Our results show that LEI-945 holds promise to guide the discovery of ALDH-based therapeutics
HostingRepository	PRIDE
AnnounceDate	2019-12-19
AnnouncementXML	Submission_2020-01-07_06:59:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bogdan Florea
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-18 03:37:12	ID requested
1	2019-12-19 02:06:11	announced
⏵ 2	2020-01-07 06:59:09	announced	2020-01-07: Updated publication reference for PubMed record(s): 31893226.

Koenders STA, Wijaya LS, Erkelens MN, Bakker AT, van der Noord VE, van Rooden EJ, Burggraaff L, Putter PC, Botter E, Wals K, van den Elst H, den Dulk H, Florea BI, van de Water B, van Westen GJP, Mebius RE, Overkleeft HS, Le D, é, v, é, dec SE, van der Stelt M, Development of a Retinal-Based Probe for the Profiling of Retinaldehyde Dehydrogenases in Cancer Cells. ACS Cent Sci, 5(12):1965-1974(2019) [pubmed]

submitter keyword: Human, cancer, aldehyde dehydrogenases, activity-based protein profiling

Mario van der Stelt, PhD
contact affiliation	Molecular Physiology, Leiden Institute for Chemistry, Leiden University, The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Bogdan Florea
contact affiliation	Leiden Institute for Chemistry
contact email	b.florea@chem.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015495

PRIDE project URI

• PRIDE
  1. PXD015495
     1. Label: PRIDE project
     2. Name: Profiling of retinaldehyde dehydrogenases in cancer cells