PXD015491

PXD015491 is an original dataset announced via ProteomeXchange.

Title	G3BP1-linked mRNA partitioning supports selective protein synthesis in response to oxidative stress
Description	Cells limit energy-consuming mRNA translation during stress to maintain metabolic homeostasis. Sequestration of mRNAs by RNA binding proteins (RBPs) into stress granules (SGs) reduces translation, but it remains unclear whether SGs also function in partitioning of specific transcripts to polysomes (PSs) to guide selective translation and stress-adaptation in cancer. Transcripts enriched in PSs, defined by polysome fractionation and RNAseq, were compared with mRNAs complexed with the SG-nucleator protein, G3BP1, defined by spatially-restricted enzymatic tagging. Short-term oxidative stress profoundly altered mRNA translation by promoting selective enrichment of transcripts within SGs or PSs. G3BP1 participates in this compartmentalisation by sequestering transcripts in SGs. Under stress, G3BP1-bound transcripts are PS-depleted and encode proteins involved in mRNA translation, pro-apoptosis, and mitochondrial function. In contrast, specific PS-enriched transcripts disassociate from G3BP1 under stress to encode proteins involved in diverse cellular cytoprotective pathways. Therefore, G3BP1 partitioning guides selective translation to support stress adaptation and cell survival.
HostingRepository	PRIDE
AnnounceDate	2020-04-30
AnnouncementXML	Submission_2020-07-15_01:02:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Syam Prakash Somasekharan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-18 01:28:53	ID requested
1	2020-04-29 21:59:40	announced
⏵ 2	2020-07-15 01:02:55	announced	2020-07-15: Updated publication reference for PubMed record(s): 32406909.

Somasekharan SP, Zhang F, Saxena N, Huang JN, Kuo IC, Low C, Bell R, Adomat H, Stoynov N, Foster L, Gleave M, Sorensen PH, G3BP1-linked mRNA partitioning supports selective protein synthesis in response to oxidative stress. Nucleic Acids Res, 48(12):6855-6873(2020) [pubmed]

submitter keyword: G3BP1, APEX, Prostate cancer, Stress granules

Syam Prakash Somasekharan
contact affiliation	Vancouver Prostate Centre Vancouver, BC, Canada
contact email	syamprakash@gmail.com
lab head
Syam Prakash Somasekharan
contact affiliation	Vancouver Prostate Centre
contact email	syamprakash@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015491

PRIDE project URI

• PRIDE
  1. PXD015491
     1. Label: PRIDE project
     2. Name: G3BP1-linked mRNA partitioning supports selective protein synthesis in response to oxidative stress