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PXD015490

PXD015490 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis identifies IRSp53 and Fascin as critical for PRV egress and direct cell-cell transmission
DescriptionPseudorabies virus (PRV) has been widely used as a live transsynaptic tracer for mapping neuronal circuits. Systematically identifying mature PRV virion proteomes and defining copurified host proteins are necessary to fully understand the detailed mechanism underlying PRV transmission processes. Here, we developed a PRV virion purification strategy based on sorting with flow cytometry and characterized the mature extracellular and intracellular PRV virion proteomes using liquid chromatography coupled with tandem mass spectrometry. In addition to viral proteins, a large number of host proteins were also identified, including proteins related to actin cytoskeletal dynamics and membrane protrusion. How many of these host proteins are true virion components are unknown and the majority of these may not be. Through functional analysis, we found that IRSp53 and fascin were critical for the egress process and play a role in direct cell-cell transmission. Moreover, we showed that CDC42 and Rac1 were also involved in the production of mature extracellular virions. Our results suggest that the formation of the filopodia-like cytoskeleton and the rearrangement of the membrane, which are both associated with IRSp53 and fascin, may be important for the transmission of viruses used in neuronal tracing.
HostingRepositoryiProX
AnnounceDate2019-09-18
AnnouncementXMLSubmission_2019-12-09_21:56:26.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFei-long Yu
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-17 23:45:30ID requested
12019-09-17 23:47:01announced
22019-12-09 21:56:28announced2019-12-10: Update publication information.
Publication List
Yu FL, Miao H, Xia J, Jia F, Wang H, Xu F, Guo L, Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell-Cell Transmission. Proteomics, 19(23):e1900009(2019) [pubmed]
Keyword List
submitter keyword: Proteomics, Virus, PRV
Contact List
Lin Guo
contact affiliationWuhan University
contact emailguol@whu.edu.cn
lab head
Fei-long Yu
contact affiliationWuhan University
contact emailyfl0922@whu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI