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PXD015487

PXD015487 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleClostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach
DescriptionClostridium cellulovorans is among the most promising candidates for consolidated bioprocessing (CBP) of cellulosic biomass to liquid biofuels (e.g. ethanol, butanol). C. cellulovoranscan metabolize all the main plant polysaccharides (i.e. cellulose, hemicellusoses and pectins). Unlike other well established cellulolytic microorganisms, C. cellulovorans most abundant catabolite is butyrate. This attracted attention on this strain as potential butanol producer since most reactions involved in butyrate and butanol synthetic pathway from acetyl-coA are common. Recent studies demonstrated that the introduction of a single heterologous alcohol/aldehyde dehydrogenase can significantly divert the branching-point intermediate, i.e. butyryl-CoA, towards butanol production in this strain. In spite of the potential of C. cellulovorans for application in CBP of plant biomass, engineering its metabolic pathways towards industrial utilization still requires enhanced understanding of its metabolism. Few recent studies aimed at understanding the regulation of C. cellulovorans central carbon metabolism in response to different substrate availability, which seem insufficient for the aforementioned purposes. The present study aimed at improving comprehension of cellulose metabolism in C. cellulovorans by comparing growth kinetics, substrate consumption/product accumulation and whole-cell soluble proteome with those of C. cellulovorans grown on a soluble carbohydrate, i.e. glucose, as the main carbon source. Modulations of the central carbon metabolism in response to changes in the growth substrate were detected, including the regulation of glycolytic enzymes, fermentation pathways and nitrogen assimilation. Our data suggest that a higher energy expenditure occurs in cellulose-grown C. cellulovorans which induces up-regulation of ATP synthetic pathways, e.g. acetate production and ATP synthase.
HostingRepositoryPRIDE
AnnounceDate2020-01-30
AnnouncementXMLSubmission_2020-01-30_00:26:47.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarcello Manfredi
SpeciesList scientific name: Clostridium cellulovorans 743B; NCBI TaxID: 573061;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-17 05:30:07ID requested
12020-01-30 00:40:34announced
Publication List
Usai G, Cirrincione S, Re A, Manfredi M, Pagnani A, Pessione E, Mazzoli R, Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach. J Proteomics, 216():103667(2020) [pubmed]
Keyword List
submitter keyword: ATP, acetate, ethanol, Alcohol dehydrogenase, pyruvate phosphate dikinase, glucose
Contact List
Marcello Manfredi
contact affiliationProteomics and Metabolomics Lab, Department of Translational Medicine (DiMeT), Center for Translational Research on Autoimmune & Allergic Diseases - CAAD, University of Piemonte Orientale, Novara (NO), Italy
contact emailmarcello.manfredi@uniupo.it
lab head
Marcello Manfredi
contact affiliationUniversity of Eastern Piedmont
contact emailmarcello.manfredi@uniupo.it
dataset submitter
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Dataset FTP location
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