PXD015487 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach |
Description | Clostridium cellulovorans is among the most promising candidates for consolidated bioprocessing (CBP) of cellulosic biomass to liquid biofuels (e.g. ethanol, butanol). C. cellulovoranscan metabolize all the main plant polysaccharides (i.e. cellulose, hemicellusoses and pectins). Unlike other well established cellulolytic microorganisms, C. cellulovorans most abundant catabolite is butyrate. This attracted attention on this strain as potential butanol producer since most reactions involved in butyrate and butanol synthetic pathway from acetyl-coA are common. Recent studies demonstrated that the introduction of a single heterologous alcohol/aldehyde dehydrogenase can significantly divert the branching-point intermediate, i.e. butyryl-CoA, towards butanol production in this strain. In spite of the potential of C. cellulovorans for application in CBP of plant biomass, engineering its metabolic pathways towards industrial utilization still requires enhanced understanding of its metabolism. Few recent studies aimed at understanding the regulation of C. cellulovorans central carbon metabolism in response to different substrate availability, which seem insufficient for the aforementioned purposes. The present study aimed at improving comprehension of cellulose metabolism in C. cellulovorans by comparing growth kinetics, substrate consumption/product accumulation and whole-cell soluble proteome with those of C. cellulovorans grown on a soluble carbohydrate, i.e. glucose, as the main carbon source. Modulations of the central carbon metabolism in response to changes in the growth substrate were detected, including the regulation of glycolytic enzymes, fermentation pathways and nitrogen assimilation. Our data suggest that a higher energy expenditure occurs in cellulose-grown C. cellulovorans which induces up-regulation of ATP synthetic pathways, e.g. acetate production and ATP synthase. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-30 |
AnnouncementXML | Submission_2020-01-30_00:26:47.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marcello Manfredi |
SpeciesList | scientific name: Clostridium cellulovorans 743B; NCBI TaxID: 573061; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-17 05:30:07 | ID requested | |
⏵ 1 | 2020-01-30 00:40:34 | announced | |
Publication List
Usai G, Cirrincione S, Re A, Manfredi M, Pagnani A, Pessione E, Mazzoli R, Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach. J Proteomics, 216():103667(2020) [pubmed] |
Keyword List
submitter keyword: ATP, acetate, ethanol, Alcohol dehydrogenase, pyruvate phosphate dikinase, glucose |
Contact List
Marcello Manfredi |
contact affiliation | Proteomics and Metabolomics Lab, Department of Translational Medicine (DiMeT), Center for Translational Research on Autoimmune & Allergic Diseases - CAAD, University of Piemonte Orientale, Novara (NO), Italy |
contact email | marcello.manfredi@uniupo.it |
lab head | |
Marcello Manfredi |
contact affiliation | University of Eastern Piedmont |
contact email | marcello.manfredi@uniupo.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015487
- Label: PRIDE project
- Name: Clostridium cellulovorans metabolism of cellulose as studied by comparative proteomic approach