PXD015484

PXD015484 is an original dataset announced via ProteomeXchange.

Title	Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast
Description	Constitutive domains of repressive heterochromatin are maintained within the fission yeast genome through self-reinforcing mechanisms involving histone methylation and small RNAs. Non-coding RNAs generated from heterochromatic regions are processed into small RNAs by the RNA interference pathway, and are subject to silencing through both transcriptional and post-transcriptional mechanisms. While the pathways involved in maintenance of the repressive heterochromatin state are reasonably well understood, less is known about the requirements for its establishment. Here we describe a novel role for the post-transcriptional regulatory factor Mkt1 in establishment of heterochromatin at pericentromeres in fission yeast. Loss of Mkt1 does not affect maintenance of existing heterochromatin, but does affect its recovery following depletion, as well as de novo establishment of heterochromatin on a mini-chromosome. Pathway dissection revealed that Mkt1 is required for RNAi-mediated post-transcriptional silencing, downstream of small RNA production. Mkt1 physically associates with pericentromeric transcripts, and is additionally required for maintenance of silencing and heterochromatin at centromeres when transcriptional silencing is impaired. Our findings provide new insight into the mechanism of RNAi-mediated post-transcriptional silencing in fission yeast, and unveil an important role for post-transcriptional silencing in establishment of heterochromatin that is dispensable when full transcriptional silencing is imposed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:57:33.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizabeth Bayne
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-17 02:39:50	ID requested
1	2019-11-28 07:48:10	announced
2	2020-01-14 07:45:07	announced	2020-01-14: Updated publication reference for PubMed record(s): 31822915.
⏵ 3	2024-10-22 04:57:41	announced	2024-10-22: Updated project metadata.

10.1093/nar/gkz1157;

Taglini F, Chapman E, van Nues R, Theron E, Bayne EH, Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast. Nucleic Acids Res, 48(3):1239-1253(2020) [pubmed]

submitter keyword: Heterochromatin, Schizosaccharomyces pombe, RNA interference

Elizabeth Bayne
contact affiliation	Unioversity of Edinburgh
contact email	elizabeth.bayne@ed.ac.uk
lab head
Elizabeth Bayne
contact affiliation	University of Edinburgh
contact email	elizabeth.bayne@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015484

PRIDE project URI

• PRIDE
  1. PXD015484
     1. Label: PRIDE project
     2. Name: Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast