PXD015480

PXD015480 is an original dataset announced via ProteomeXchange.

Title	The effects of PhACL silencing on protein acetylation in petunia the effects of acetyl-CoA
Description	Acetyl-coenzyme A (acetyl-CoA) is a central metabolite and the acetyl source for protein acetylation. The formation of cytosolic acetyl-CoA is catalyzed by ATP-citrate lyase (ACL) from citrate. However, the effects of acetyl-CoA on protein acetylation were not well known. In this study, four genes, PhACLA1, PhACLA2, PhACLB1, PhACLB2, encoding ACLA and ACLB submits in petunia (Petunia hybrida) were identified. VIGS-mediated PhACLA1-A2 and PhACLB1-B2 silencing leads to abnormal leave and flower development and reduced total anthocyanins content, while silencing of any single PhACL gene did not result in visible phenotype change. We quantitatively investigated the petunia proteome, acetylome, and the association between them in petunia corollas treated with pTRV2-PhACLB1-B2 and pTRV2. In total, 6200 protein groups were identified from petunia, among which 5343 proteins were quantified. A total of 345 proteins were up-regulated and 182 proteins were down-regulated (with a threshold of 1.2-fold) in PhACLB1-B2-silenced plant compared with the control (P <0.05). A total of 2210 lysine acetylation sites in 1148 protein groups were identified, among which 1744 sites in 921 proteins were accurately quantified (Additional file 2: Table S7). We subsequently used the quantification results of the global proteome to normalize the acetylome quantification data. From these, 68 sites in 54 lysine acetylation proteins were quantified as up-regulated targets and 40 sites in 38 lysine acetylation proteins were quantified as down-regulated targets at a threshold of 1.2 (P < 0.05). The global proteome and acetylome were negatively correlated, implying that proteome expression levels were negatively regulated by acetylation in PhACLB1-B2-silenced plants compared with the control.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:41.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yixun Yu
SpeciesList	scientific name: Petunia hybrida (Petunia); NCBI TaxID: 4102;
ModificationList	acetylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-17 02:38:54	ID requested
1	2020-06-23 22:13:12	announced
⏵ 2	2024-10-22 05:07:44	announced	2024-10-22: Updated project metadata.

10.1093/jxb/eraa208;

Zhao H, Zhong S, Sang L, Zhang X, Chen Z, Wei Q, Chen G, Liu J, Yu Y, PaACL silencing accelerates flower senescence and changes the proteome to maintain metabolic homeostasis in Petunia hybrida. J Exp Bot, 71(16):4858-4876(2020) [pubmed]

submitter keyword: Petunia

ACL

Metabolome

Acetylome

Yixun Yu
contact affiliation	South China Agricultural University
contact email	yuyixun@scau.edu.cn
lab head
Yixun Yu
contact affiliation	Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	yuyixun@scau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD015480

PRIDE project URI

• PRIDE
  1. PXD015480
     1. Label: PRIDE project
     2. Name: The effects of PhACL silencing on protein acetylation in petunia the effects of acetyl-CoA