PXD015476

PXD015476 is an original dataset announced via ProteomeXchange.

Title	Yeast homologs of MCUR1 regulate mitochondrial proline metabolism
Description	MCUR1 is an evolutionarily conserved mitochondrial protein that has been shown to act as a scaffold factor for the assembly of the mammalian mitochondrial calcium uniporter (MCU). Surprisingly, many fungi that do not have MCU contain MCUR1 homologs, suggesting these proteins possess alternate functions. Herein we characterized two Saccharomyces cerevisiae homologs of MCUR1- Put6 and Put7 as regulators of mitochondrial proline metabolism. Put6 and Put7 are tethered to the inner mitochondrial membrane in a large hetero-oligomeric complex. The abundance and assembly of this complex are regulated by proline and the loss of this complex results in unregulated proline uptake in the mitochondria, leading to redox imbalance in the cell. Yeast cells lacking either Put6 or Put7 exhibit a pronounced defect in proline utilization, which can be corrected by the heterologous expression of human MCUR1. Our work uncovers an unexpected role of evolutionarily conserved MCUR1 homologs in mitochondrial proline metabolism.
HostingRepository	PRIDE
AnnounceDate	2020-09-30
AnnouncementXML	Submission_2020-09-29_23:32:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Murgia
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-17 01:54:03	ID requested
⏵ 1	2020-09-29 23:32:02	announced

Zulkifli M, Neff JK, Timbalia SA, Garza NM, Chen Y, Watrous JD, Murgia M, Trivedi PP, Anderson SK, Tomar D, Nilsson R, Madesh M, Jain M, Gohil VM, Yeast homologs of human MCUR1 regulate mitochondrial proline metabolism. Nat Commun, 11(1):4866(2020) [pubmed]

curator keyword: Biological

submitter keyword: mitochondrion, proline metabolism, MCUR1

Vishal M. Gohil
contact affiliation	Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA
contact email	vgohil@tamu.edu
lab head
Marta Murgia
contact affiliation	MPI Biochemistry
contact email	mmurgia@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015476
     1. Label: PRIDE project
     2. Name: Yeast homologs of MCUR1 regulate mitochondrial proline metabolism