PXD015475

PXD015475 is an original dataset announced via ProteomeXchange.

Title	The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant
Description	A century ago, influenza A virus (IAV) infection caused the 1918 flu pandemic and killed an estimated 20-40 million people. Pandemic IAV outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular details of this species barrier are incompletely understood. We combined metabolic pulse labeling and quantitative shotgun proteomics to globally monitor protein synthesis upon infection of human cells with a human- and a bird-adapted IAV strain. While production of host proteins was remarkably similar, we observed striking differences in the kinetics of viral protein synthesis over the course of infection. Most importantly, the matrix protein M1 was inefficiently produced by the bird-adapted strain at later stages. We show that impaired production of M1 from bird-adapted strains is caused by increased splicing of the M segment RNA to alternative isoforms. Experiments with reporter constructs and recombinant influenza viruses revealed that strain-specific M segment splicing is controlled by the 3’ splice site and functionally important for permissive infection. Independent in silico and biochemical evidence shows that avian-adapted M segments have evolved different conserved RNA structure features than human-adapted sequences. Thus, our data identifies M segment RNA splicing as a viral determinant of host range.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:57:40.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boris Bogdanow
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 23:18:54	ID requested
1	2019-11-11 06:39:12	announced
2	2019-11-27 06:01:09	announced	2019-11-27: Updated project metadata.
3	2020-01-13 05:25:11	announced	2019-11-27: Updated project metadata. 2020-01-13: Updated publication reference for PubMed record(s): 31797923.
⏵ 4	2024-10-22 04:57:48	announced	2024-10-22: Updated project metadata.

Bogdanow B, Wang X, Eichelbaum K, Sadewasser A, Husic I, Paki K, Budt M, Hergeselle M, Vetter B, Hou J, Chen W, Wiebusch L, Meyer IM, Wolff T, Selbach M, The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant. Nat Commun, 10(1):5518(2019) [pubmed]

10.1038/s41467-019-13520-8;

curator keyword: Biomedical

submitter keyword: influenza a virus infection

Matthias Selbach
contact affiliation	Max-Delbrück Center for Molecular Medicine
contact email	matthias.selbach@mdc-berlin.de
lab head
Boris Bogdanow
contact affiliation	MDC Berlin
contact email	boris.bogdanow@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015475

PRIDE project URI

• PRIDE
  1. PXD015475
     1. Label: PRIDE project
     2. Name: The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant