PXD015471

PXD015471 is an original dataset announced via ProteomeXchange.

Title	The mRNA-binding proteome of proliferating and differentiated muscle cells
Description	Muscle formation is a coordinated process driven by extensive gene expression changes where single cells fuse together to form multinucleated muscle fibers. Newly synthesized mRNAs are then regulated by RNA binding proteins(RBPs)affecting post-transcriptional transcripts metabolism. We determined how large gene expression changes affect the catalog of RBPs by studying proliferating and differentiated muscle cells in healthy and dystrophic conditions. Transcriptomic analysis showed that the expression of more than 7000 genes was affected during myogenesis. We identified 769 RBPs, of which 294 were muscle specific and 49 were uniquely shared with cardiomyocytes. A subset of 32 RBPs (half of which was muscle specific) showed preferential loading on target mRNAs in either myoblasts or myotubes. A large proportion of catalytic proteins was bound to mRNAs even though they lack classical RNA binding domains. Finally we show how the identification of cell specific RBPs enabled the identification biomarkers that can separate healthy individuals from dystrophic patients. Our data show how interactome data can shed light on new basic RNA biology as well as provide cell specific data that can be used for diagnostic purposes.
HostingRepository	PRIDE
AnnounceDate	2020-06-09
AnnouncementXML	Submission_2020-06-08_17:00:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	G Janssen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 07:30:54	ID requested
⏵ 1	2020-06-08 17:00:49	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: RNA binding proteins

Interactome

Duchenne muscular dystrophy

P.A.van Veelen
contact affiliation	Centre for Proteomics and Metabolomics Head Proteomics group LUMC
contact email	P.A.van_Veelen@lumc.nl
lab head
G Janssen
contact affiliation	Leiden University Medical Center
contact email	g.m.c.janssen@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD015471

PRIDE project URI

• PRIDE
  1. PXD015471
     1. Label: PRIDE project
     2. Name: The mRNA-binding proteome of proliferating and differentiated muscle cells