PXD015469

PXD015469 is an original dataset announced via ProteomeXchange.

Title	Proteomics Analysis of the Mechanism of AMP-17 against Candida albicans
Description	Candida albicans is the most common human fungal pathogen in immunocompromised individuals. With the emergence of clinical fungal resistance, there is an urgent demand to develop novel antifungal agents. The antibacterial peptide (AMP) is an important component in the innate immune system and has a good therapeutic effect on patients infected with microorganisms. AMP-17, a novel AMP from Musca domestica, has an antifungal effect against C. albicans, but the mechanism of antifungal action remains unclear. In this study, we performed proteomic analysis of C. albicans treated AMP-17 and No drug using a combination of a series of cutting-edge technologies, including TMT labeling, HPLC classification and quantitative proteomics based on mass spectrometry. A total of 3,931 proteins were identified, of which 3,600 contained quantitative information. With a 1.5-fold change threshold and a t-test p-value <0.05 as a standard, 423 differentially expressed proteins (DEPs) were up-regulated and 180 differentially expressed proteins (DEPs) were down-regulated in the quantitative AMP_17/con comparison group. In these DEPs, proteins associated with ergosterol biosynthesis, oxidative stress and cell wall were identified as significantly up-regulated, while proteins involved in fatty acid biosynthesis were identified as significantly down-regulated. In addition, using the KEGG enrichment assay, seven significant KEGG pathways were identified, primarily involved in oxidative phosphorylation, propionic acid metabolism, and fatty acid metabolism. These results showed that AMP-17 induces a complex organism response to C. albicans, indicating that AMP-17 can inhibit growth by affecting multiple targets in C. albicans cells.
HostingRepository	PRIDE
AnnounceDate	2019-10-23
AnnouncementXML	Submission_2019-10-23_09:26:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	guo Guo
SpeciesList	scientific name: Candida albicans; NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 05:43:40	ID requested
⏵ 1	2019-10-23 09:26:27	announced

Dataset with its publication pending

submitter keyword: Candida albicans,Antimicrobial peptides,AMP-17,Mechanism,Proteomic,TMT- Labeling

Guo Guo
contact affiliation	Building Wuben,School of Basic Medical Sciences,Guizhou Medical University,College Town,Gui’an New District,Guiyang,550025,China
contact email	guoguojsc@163.com
lab head
guo Guo
contact affiliation	chool of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550025, China
contact email	guoguojsc@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015469
     1. Label: PRIDE project
     2. Name: Proteomics Analysis of the Mechanism of AMP-17 against Candida albicans