PXD015466

PXD015466 is an original dataset announced via ProteomeXchange.

Title	Interactome of AMPK complex study using TAP-MS
Description	The adenosine monophosphate-activated protein kinase (AMPK) complex is a key enzyme in regulating cellular energy homeostasis. This complex is well studied and it is known to function in many cellular pathways. However, the interactome of AMPK complex has not yet been systematically established, although protein-protein interaction is critically important for protein function and regulation. Here, we applied tandem-affinity purification coupled with mass spectrometry (TAP-MS) strategy to study the AMPK complex interactome to discover its new functions. We conducted TAP-MS for all the seven AMPK complex subunits. The control TAP-MS group in the dataset includes forty-six control TAP-MS results using non-related baits. HEK293T proteome profiling results were used as background controls in HCIPs analysis. With these analysis, we identified 145 high confident interacting proteins (HCIPs) of AMPK, which allowed us to build an interaction network of AMPK complex.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:59:46.425.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 05:42:44	ID requested
1	2020-01-09 00:58:22	announced
⏵ 2	2024-10-22 04:59:53	announced	2024-10-22: Updated project metadata.

Chen Z, Wang C, Jain A, Srivastava M, Tang M, Zhang H, Feng X, Nie L, Su D, Xiong Y, Jung SY, Qin J, Chen J, AMPK Interactome Reveals New Function in Non-homologous End Joining DNA Repair. Mol Cell Proteomics, 19(3):467-477(2020) [pubmed]

10.1074/mcp.ra119.001794;

submitter keyword: Interactome,AMPK, TAP

Junjie Chen
contact affiliation	Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA
contact email	JChen8@mdanderson.org
lab head
Zhen Chen
contact affiliation	The University of Texas MD Anderson Cancer Center
contact email	zchen6@mdanderson.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015466

PRIDE project URI

• PRIDE
  1. PXD015466
     1. Label: PRIDE project
     2. Name: Interactome of AMPK complex study using TAP-MS