PXD015458

PXD015458 is an original dataset announced via ProteomeXchange.

Title	Tissue Thermal Proteome Profiling
Description	We introduce tissue Thermal Proteome Profiling (tissue-TPP) which enables the discovery of drug targets and off-targets as well as measurements of target occupancy in tissue samples derived from dosed animals. Here we report the first proteome-wide thermal stability map of tissues and organ-specific target profiles of the non-covalent histone deacetylase inhibitor, panobinostat. In addition, we devised blood-CETSA/TPP to monitor target and off-target engagement directly in whole blood.
HostingRepository	PRIDE
AnnounceDate	2020-01-07
AnnouncementXML	Submission_2020-02-17_02:22:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nils Kurzawa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 01:14:12	ID requested
1	2020-01-07 11:20:45	announced
⏵ 2	2020-02-17 02:22:09	announced	2020-02-17: Updated publication reference for PubMed record(s): 31959954.

Perrin J, Werner T, Kurzawa N, Rutkowska A, Childs DD, Kalxdorf M, Poeckel D, Stonehouse E, Strohmer K, Heller B, Thomson DW, Krause J, Becher I, Eberl HC, Vappiani J, Sevin DC, Rau CE, Franken H, Huber W, Faelth-Savitski M, Savitski MM, Bantscheff M, Bergamini G, Identifying drug targets in tissues and whole blood with thermal-shift profiling. Nat Biotechnol, 38(3):303-308(2020) [pubmed]

curator keyword: Technical

submitter keyword: TPP, Mouse, Liver

Marcus Bantscheff
contact affiliation	Cellzome, a GSK company
contact email	marcus.x.bantscheff@gsk.com
lab head
Nils Kurzawa
contact affiliation	EMBL Heidelberg
contact email	nils.kurzawa@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015458

PRIDE project URI

• PRIDE
  1. PXD015458
     1. Label: PRIDE project
     2. Name: Tissue Thermal Proteome Profiling