PXD015457

PXD015457 is an original dataset announced via ProteomeXchange.

Title	Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome
Description	In the presented datasets, we use newly developed isotopically labeled desthiobiotin azide (isoDTB) tags to residue-specifically quantify cysteines in a variety of bacterial strains and a human cell line with consistently high coverage. We e.g. quantify 59% of all cysteines in essential proteins in Staphylococcus aureus. We discover 88 cysteines with high reactivity, which correlates with functional importance. Furthermore, we identify 268 cysteines that are engaged by covalent ligands. Overall, a comprehensive map of the bacterial cysteinome is obtained.
HostingRepository	PRIDE
AnnounceDate	2019-12-02
AnnouncementXML	Submission_2019-12-02_10:34:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan M. Hacker
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 01:13:04	ID requested
⏵ 1	2019-12-02 10:34:42	announced

Zanon PRA, Lewald L, Hacker SM, Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome. Angew Chem Int Ed Engl, 59(7):2829-2836(2020) [pubmed]

curator keyword: Technical

submitter keyword: Antibiotics, Covalent Inhibitors, Isotopic labeling, Protein modifications, Proteomics

Dr. Stephan M. Hacker
contact affiliation	Department of Chemistry Technical University of Munich Germany
contact email	stephan.m.hacker@tum.de
lab head
Stephan M. Hacker
contact affiliation	Technical University of Munich
contact email	stephan.m.hacker@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015457

PRIDE project URI

• PRIDE
  1. PXD015457
     1. Label: PRIDE project
     2. Name: Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome