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PXD015457 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome
DescriptionIn the presented datasets, we use newly developed isotopically labeled desthiobiotin azide (isoDTB) tags to residue-specifically quantify cysteines in a variety of bacterial strains and a human cell line with consistently high coverage. We e.g. quantify 59% of all cysteines in essential proteins in Staphylococcus aureus. We discover 88 cysteines with high reactivity, which correlates with functional importance. Furthermore, we identify 268 cysteines that are engaged by covalent ligands. Overall, a comprehensive map of the bacterial cysteinome is obtained.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephan M. Hacker
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-16 01:13:04ID requested
12019-12-02 10:34:42announced
Publication List
Zanon PRA, Lewald L, Hacker SM, Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome. Angew Chem Int Ed Engl, ():(2019) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Antibiotics, Covalent Inhibitors, Isotopic labeling, Protein modifications, Proteomics
Contact List
Dr. Stephan M. Hacker
contact affiliationDepartment of Chemistry Technical University of Munich Germany
contact emailstephan.m.hacker@tum.de
lab head
Stephan M. Hacker
contact affiliationTechnical University of Munich
contact emailstephan.m.hacker@tum.de
dataset submitter
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