PXD015452

PXD015452 is an original dataset announced via ProteomeXchange.

Title	Multiomics Analyses Reveal Dynamic Bioenergetic Pathways and Functional Remodeling of the Heart During Intermittent Fasting
Description	Intermittent fasting (IF) reduces cardiovascular risk factors in animals and humans, and can protect the heart against ischemic injury in models of myocardial infarction, but the underlying molecular mechanisms are unknown. To delineate molecular perturbations in response to IF, we carried out comprehensive analyses of molecular pathways and biological processes using proteomic and phosphoproteomic data followed by functional analysis of hearts from mice maintained for 6 months on either daily 12- or 16-hour fasting, every-other-day fasting or ad libitum control feeding regimens. IF regimens significantly affected pathways that regulate cyclic GMP signaling, lipid and amino acid metabolism, cell adhesion, cell death and inflammation. Comparison of differentially expressed proteome and transcriptome upon IF showed higher correlation of pathway alternation in early IF regimen, but inverse correlation of metabolic processes such as fatty acid oxidation and immune processes in later IF regimens. Echocardiographic analyses demonstrated that IF enhances stress-induced cardiac performance. In addition to providing a valuable resource, our systemic analyses reveal molecular framework for understanding how IF impacts the function of the heart and its vulnerability to injury and disease.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:56.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Asfa Alli Shaik
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 01:10:19	ID requested
1	2023-09-27 02:25:00	announced
⏵ 2	2023-11-14 08:24:56	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Intermittent Fasting, Heart, Proteome, Mice, Phosphoproteome, Transcriptome

Jayantha GUNARATNE
contact affiliation	Institute of Molecular and Cell Biology, A*STAR, Singapore
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Asfa Alli Shaik
contact affiliation	IMCB
contact email	asfaas@imcb.a-star.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015452
     1. Label: PRIDE project
     2. Name: Multiomics Analyses Reveal Dynamic Bioenergetic Pathways and Functional Remodeling of the Heart During Intermittent Fasting