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PXD015447 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFeS protein levels in Arabidopsis plants expressing a mutated NEET protein
DescriptionIron-sulfur (Fe-S) clusters play an essential role in plants as protein co-factors mediating diverse electron transfer reactions. Because they can react with oxygen to form reactive oxygen species (ROS) and cause cellular damage, the biogenesis of Fe-S clusters is highly regulated. A newly discovered group of 2Fe-2S proteins, termed NEET proteins, was recently proposed to coordinate iron-sulfur, iron, and ROS homeostasis in mammalian cells. Here we report that disrupting the function of AtNEET, the sole member of the NEET protein family in Arabidopsis thaliana, triggers a leaf-associated Fe- and Fe-S-deficiency responses, iron overload in chloroplasts (1.2-1.5 fold), chlorosis, structural damage to chloroplasts, and a high seedling mortality rate. Our findings suggest that disrupting AtNEET function disrupts the transfer of 2Fe-2S clusters from the chloroplastic 2Fe-2S biogenesis pathway to Fe-S proteins, and that uncoupling this process triggers a leaf-associated Fe-deficiency response that results in iron overload in chloroplasts and enhanced ROS accumulation
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSara I Zandalinas
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentTSQ Quantiva
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-13 12:22:53ID requested
12021-01-24 16:20:41announced
Publication List
Zandalinas SI, Song L, Sengupta S, McInturf SA, Grant DG, Marjault HB, Castro-Guerrero NA, Burks D, Azad RK, Mendoza-Cozatl DG, Nechushtai R, Mittler R, Expression of a dominant-negative AtNEET-H89C protein disrupts iron-sulfur metabolism and iron homeostasis in Arabidopsis. Plant J, 101(5):1152-1169(2020) [pubmed]
Keyword List
submitter keyword: Arabidopsis, NEET, Fe-S
Contact List
Ron Mittler
contact affiliationUniversity of Missouri
contact emailmittlerr@missouri.edu
lab head
Sara I Zandalinas
contact affiliationUniversity of Missouri
contact emailsara.i.zandalinas@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI