PXD015443

PXD015443 is an original dataset announced via ProteomeXchange.

Title	Dynamic changes in the brain protein interaction network correlates with progression of Aβ42 pathology in Drosophila
Description	Alzheimer’s disease (AD), the most prevalent form of dementia, is a progressive and devastating neurodegenerative condition for which there are no effective treatments. Understanding the molecular pathology of AD during disease progression may identify new ways to reduce neuronal damage. Here, we present a longitudinal study tracking dynamic proteomic alterations in the brains of an inducible Drosophila melanogastermodel of AD expressing the Arctic mutant Aβ42 gene. We identified 3093 proteins from flies that were induced to express Aβ42 and age-matched healthy controls using label-free quantitative ion-mobility data independent analysis mass spectrometry. Of these, 228 proteins were significantly altered by Aβ42 accumulation and were enriched for AD-associated processes. Network analyses further revealed that these proteins have distinct hub and bottleneck properties in the brain protein interaction network, suggesting that several may have significant effects on brain function. Our unbiased analysis provides useful insights into the key processes governing the progression of amyloid toxicity and forms a basis for further functional analyses in model organisms and translation to mammalian systems
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:21.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Menneteau
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized amino-terminal residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-13 03:40:08	ID requested
1	2020-12-08 08:11:45	announced
⏵ 2	2024-10-22 05:16:25	announced	2024-10-22: Updated project metadata.

Scholes HM, Cryar A, Kerr F, Sutherland D, Gethings LA, Vissers JPC, Lees JG, Orengo CA, Partridge L, Thalassinos K, 42 pathology in Drosophila. Sci Rep, 10(1):18517(2020) [pubmed]

10.1038/s41598-020-74748-9;

submitter keyword: brain,Drosophila, Aβ42 pathology, interaction network

Konstantinos Thalassinos
contact affiliation	Institute of Structural and Molecular Biology, University College London, London, United Kingdom
contact email	k.thalassinos@ucl.ac.uk
lab head
Thomas Menneteau
contact affiliation	Institute of Structural and Molecular Biology - UCL
contact email	t.menneteau@ucl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD015443

PRIDE project URI

• PRIDE
  1. PXD015443
     1. Label: PRIDE project
     2. Name: Dynamic changes in the brain protein interaction network correlates with progression of Aβ42 pathology in Drosophila