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PXD015437

PXD015437 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTULIP2: an improved method for the identification of ubiquitin E3-specific targets
DescriptionProtein modification by Ubiquitin or Ubiquitin-like modifiers is mediated by an enzyme cascade composed of E1s, E2s and E3s enzymes. E1, or ubiquitin-activating enzymes, perform the ubiquitin activation. Next, ubiquitin is transferred to ubiquitin-conjugating enzymes or E2s. Finally, ubiquitin ligases or E3s catalyze the transfer of ubiquitin to the acceptor proteins. E3 enzymes are responsible of determining the substrate specificity. Determining which E3 enzyme maps to which substrate is a major challenge that is greatly facilitated by the TULIP2 methodology. TULIP2 methodology is fast, precise and cost-effective. Compared to previous TULIP methodology protocol, TULIP2 methodology achieves a more than 50-fold improvement in the purification yield and two orders of magnitude improvement in the signal-to-background ratio after label free quantification by mass spectrometry analysis. The method includes the generation of TULIP2 cell lines, subsequent purification of TULIP2 conjugates, preparation and analysis of samples by mass spectrometry
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:58:39.751.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRomán González-Prieto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-12 08:50:05ID requested
12019-11-15 05:39:55announced
22020-01-02 07:56:37announced2020-01-02: Updated publication reference for PubMed record(s): 31850303.
32024-10-22 04:58:41announced2024-10-22: Updated project metadata.
Publication List
Salas-Lloret D, Agabitini G, Gonz, á, lez-Prieto R, TULIP2: An Improved Method for the Identification of Ubiquitin E3-Specific Targets. Front Chem, 7():802(2019) [pubmed]
10.3389/fchem.2019.00802;
Keyword List
submitter keyword: Ubiquitin, E3 enzymes, Mass Spectrometry, Post-translational modifications, Proteomics
Contact List
Román González-Prieto
contact affiliationGonzález-Prieto laboratory, Department of Cell and Chemical Biology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
contact emailR.Gonzalez_Prieto@lumc.nl
lab head
Román González-Prieto
contact affiliationAndalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact emailR.Gonzalez_Prieto@lumc.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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