PXD015435 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry study of formaldehyde cross-linking of structured proteins |
Description | Formaldehyde is a widely used fixative in biology and medicine. The current chemical model for formaldehyde cross-linking of proteins is the formation of a methylene bridge that incorporates one carbon atom into the link. Here, we present mass spectrometry data that largely refute this model. Instead, our data show that cross-linking of structured proteins mainly involves a reaction that incorporates two carbon atoms into the link. Under MS/MS fragmentation, the link cleaves symmetrically to yield unusual fragments with a modification of one carbon atom. We apply this new understanding of the underlying cross-linking chemistry to the structural approach of cross-linking coupled to mass spectrometry. First, we cross-linked a mixture of purified proteins with formaldehyde. Our new analysis readily identified tens of cross-links from these proteins, which fit well with their atomic structures. We then perform in-situ cross-linking of human cells in culture. We identified 469 intra-protein and 90 inter-protein cross-links, which also fit well with available atomic structures. Interestingly, many of these cross-links could not be mapped onto a known structure and thus provide new structural insights. We highlight an example in which formaldehyde cross-links localize the binding site of βNAC on the ribosome. We also find several interactions of actin with auxiliary proteins. Our findings not only expand our understanding of formaldehyde reactivity and toxicity, but also clearly demonstrate how to use this potent reagent for structural studies. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-25 |
AnnouncementXML | Submission_2020-06-24_23:09:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nir Kalisman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-12 08:40:35 | ID requested | |
⏵ 1 | 2020-06-24 23:09:57 | announced | |
Publication List
Tayri-Wilk T, Slavin M, Zamel J, Blass A, Cohen S, Motzik A, Sun X, Shalev DE, Ram O, Kalisman N, Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins. Nat Commun, 11(1):3128(2020) [pubmed] |
Keyword List
submitter keyword: Cross-linking coupled to mass spectrometry, XL-MS, Actin, Nascent polypeptide-associated complex, Structural biology |
Contact List
Nir Kalisman |
contact affiliation | Dept. of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel |
contact email | nirka@mail.huji.ac.il |
lab head | |
Nir Kalisman |
contact affiliation | Dept. of Biological Chemistry, The Hebrew University of Jerusalem |
contact email | nirka@mail.huji.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD015435
- Label: PRIDE project
- Name: Mass spectrometry study of formaldehyde cross-linking of structured proteins