PXD015432

PXD015432 is an original dataset announced via ProteomeXchange.

Title	Neuronal interactome changes upon aggregation of Tau
Description	To understand at molecular level how aggregation of Tau modulates its interactions with proteins we reveal key determinant for derailing Tau protein network. By performing quantitative AP-MS we define a new set of interactors which bind Tau upon fibril formation. These interactors contain disordered regions with a unique amino acidic footprint. Such regions are enriched in positively charged Arginines which can engage aberrant binding to Tau fibrils through their guanidinium group via pi-stacking. We also find that the Hsp90 chaperone stalls formation of Tau fibrils and reshapes their abnormal interactome.
HostingRepository	PRIDE
AnnounceDate	2020-01-30
AnnouncementXML	Submission_2020-02-17_03:23:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Stucchi
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-12 08:39:43	ID requested
1	2020-01-30 02:55:04	announced
⏵ 2	2020-02-17 03:23:21	announced	2020-02-17: Updated publication reference for PubMed record(s): 31996674.

Ferrari L, Stucchi R, Konstantoulea K, van de Kamp G, Kos R, Geerts WJC, van Bezouwen LS, F, ö, rster FG, Altelaar M, Hoogenraad CC, R, ü, diger SGD, -stacking drives binding to fibrils of the Alzheimer protein Tau. Nat Commun, 11(1):571(2020) [pubmed]

submitter keyword: Tau, protein aggregation, fibrils, Hsp90, protein-protein interactions, molecular chaperones

Casper Hoogenraad
contact affiliation	Cell Biology, Faculty of Science, Utrecht University
contact email	c.hoogenraad@uu.nl
lab head
Riccardo Stucchi
contact affiliation	Utrecht University
contact email	r.stucchi@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015432

PRIDE project URI

• PRIDE
  1. PXD015432
     1. Label: PRIDE project
     2. Name: Neuronal interactome changes upon aggregation of Tau