PXD015428

PXD015428 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of exosomes released from heatstressed hepatocytes
Description	The aim of the current study was to explore the potential role of hepatocyte exosomes in HS induced liver injury and their protein expression patterns under HS. Isobaric tags for relative and absolute quantitation technology (iTRAQ) with liquid chromatography-tandem mass spectrometry analysis were applied to explore changes in the proteomic profiles of HS-hepatocyte exosomes. Compared to the control exosome group, 53 and 21 groups of proteins were identified as up- and down-regulated in the HS-exosomes. In addition, Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway verificated that regulation of programmed cell death was the top significant altered pathway. We demonstrated in vitro and in vivo the capacity of promoting programmed liver cell death and liver injury of HS hepatocyte exosomes. This study presented the first comprehensive analysis of the HS-hepatocyte exosomal proteome, confirming the induction of HS liver injury by exosomes.
HostingRepository	iProX
AnnounceDate	2019-09-12
AnnouncementXML	Submission_2019-09-12_04:43:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	(13)C labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-12 04:41:02	ID requested
⏵ 1	2019-09-12 04:43:05	announced

Dataset with its publication pending

submitter keyword: exosomes, extracellular vesicles, heat stroke, heat stress, hepatocyte

Lei Su
contact affiliation	General Hospital of Guangzhou Military Command, PLA, China
contact email	slei_icu@163.com
lab head
Yue Li
contact affiliation	General Hospital of Guangzhou Military Command, PLA, China
contact email	yueyue2229@163.com
dataset submitter

iProX dataset URI