PXD015416 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis |
Description | Here, B. subtilis was used as a model organism to investigate how cells respond and adapt to proteotoxic stress conditions. Our experiments suggested that the stringent response, caused by raised levels of the (p)ppGpp alarmone, plays a role during thermotolerance development and the heat shock response. Accordingly, our experiments revealed a rapid increase of cellular (p)ppGpp levels upon heat shock as well as salt- and oxidative stress. Strains lacking (p)ppGpp exhibited increased stress sensitivity, while raised (p)ppGpp levels conferred increased stress tolerance to heat- and oxidative stress. During thermotolerance development, stress response genes were highly up-regulated together with a concurrent transcriptional down-regulation of the rRNA, which was influenced by the second messenger (p)ppGpp and the transcription factor Spx. Remarkably, we observed that (p)ppGpp appeared to control the cellular translational capacity and that during heat stress the raised cellular levels of the alarmone were able to curb the rate of protein synthesis. Furthermore, (p)ppGpp controls the heat-induced expression of Hpf and thus the formation of translationally inactive 100S disomes. These results indicate that B. subtilis cells respond to heat-mediated protein unfolding and aggregation, not only by raising the cellular repair capacity, but also by decreasing translation involving (p)ppGpp mediated stringent response to concurrently reduce the protein load for the cellular protein quality control system. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:57:26.067.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Frese |
SpeciesList | scientific name: Bacillus subtilis GB03; NCBI TaxID: 1423687; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-11 23:31:20 | ID requested | |
1 | 2020-03-09 07:23:57 | announced | |
2 | 2020-06-12 02:37:40 | announced | 2020-06-12: Updated publication reference for PubMed record(s): 32176689. |
⏵ 3 | 2024-10-22 04:57:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Sch, ä, fer H, Beckert B, Frese CK, Steinchen W, Nuss AM, Beckstette M, Hantke I, Driller K, Sudzinov, á P, Kr, á, sn, ý L, Kaever V, Dersch P, Bange G, Wilson DN, Turgay K, The alarmones (p)ppGpp are part of the heat shock response of Bacillus subtilis. PLoS Genet, 16(3):e1008275(2020) [pubmed] |
10.1371/journal.pgen.1008275; |
Keyword List
curator keyword: Biological |
submitter keyword: Bacillus subtilis |
Heat stress response |
stringent response |
(p)ppGpp |
alarmone |
Rel |
Spx |
Contact List
Christian K. Frese |
contact affiliation | Max Planck Unit for the Science of Pathogens, Chariteplatz 1, 10117 Berlin, Germany |
contact email | frese@mpiib-berlin.mpg.de |
lab head | |
Christian Frese |
contact affiliation | Max Planck Unit for the Science of Pathogens |
contact email | frese@mpusp.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD015416 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015416
- Label: PRIDE project
- Name: The alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis