PXD015416

PXD015416 is an original dataset announced via ProteomeXchange.

Title	The alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis
Description	Here, B. subtilis was used as a model organism to investigate how cells respond and adapt to proteotoxic stress conditions. Our experiments suggested that the stringent response, caused by raised levels of the (p)ppGpp alarmone, plays a role during thermotolerance development and the heat shock response. Accordingly, our experiments revealed a rapid increase of cellular (p)ppGpp levels upon heat shock as well as salt- and oxidative stress. Strains lacking (p)ppGpp exhibited increased stress sensitivity, while raised (p)ppGpp levels conferred increased stress tolerance to heat- and oxidative stress. During thermotolerance development, stress response genes were highly up-regulated together with a concurrent transcriptional down-regulation of the rRNA, which was influenced by the second messenger (p)ppGpp and the transcription factor Spx. Remarkably, we observed that (p)ppGpp appeared to control the cellular translational capacity and that during heat stress the raised cellular levels of the alarmone were able to curb the rate of protein synthesis. Furthermore, (p)ppGpp controls the heat-induced expression of Hpf and thus the formation of translationally inactive 100S disomes. These results indicate that B. subtilis cells respond to heat-mediated protein unfolding and aggregation, not only by raising the cellular repair capacity, but also by decreasing translation involving (p)ppGpp mediated stringent response to concurrently reduce the protein load for the cellular protein quality control system.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:57:26.067.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Bacillus subtilis GB03; NCBI TaxID: 1423687;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-11 23:31:20	ID requested
1	2020-03-09 07:23:57	announced
2	2020-06-12 02:37:40	announced	2020-06-12: Updated publication reference for PubMed record(s): 32176689.
⏵ 3	2024-10-22 04:57:34	announced	2024-10-22: Updated project metadata.

Sch, ä, fer H, Beckert B, Frese CK, Steinchen W, Nuss AM, Beckstette M, Hantke I, Driller K, Sudzinov, á P, Kr, á, sn, ý L, Kaever V, Dersch P, Bange G, Wilson DN, Turgay K, The alarmones (p)ppGpp are part of the heat shock response of Bacillus subtilis. PLoS Genet, 16(3):e1008275(2020) [pubmed]

10.1371/journal.pgen.1008275;

curator keyword: Biological

submitter keyword: Bacillus subtilis

Heat stress response

stringent response

(p)ppGpp

alarmone

Rel

Spx

Christian K. Frese
contact affiliation	Max Planck Unit for the Science of Pathogens, Chariteplatz 1, 10117 Berlin, Germany
contact email	frese@mpiib-berlin.mpg.de
lab head
Christian Frese
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	frese@mpusp.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015416
     1. Label: PRIDE project
     2. Name: The alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis