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PXD015416

PXD015416 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe alarmone (p)ppGpp is part of the heat shock response of Bacillus subtilis
DescriptionHere, B. subtilis was used as a model organism to investigate how cells respond and adapt to proteotoxic stress conditions. Our experiments suggested that the stringent response, caused by raised levels of the (p)ppGpp alarmone, plays a role during thermotolerance development and the heat shock response. Accordingly, our experiments revealed a rapid increase of cellular (p)ppGpp levels upon heat shock as well as salt- and oxidative stress. Strains lacking (p)ppGpp exhibited increased stress sensitivity, while raised (p)ppGpp levels conferred increased stress tolerance to heat- and oxidative stress. During thermotolerance development, stress response genes were highly up-regulated together with a concurrent transcriptional down-regulation of the rRNA, which was influenced by the second messenger (p)ppGpp and the transcription factor Spx. Remarkably, we observed that (p)ppGpp appeared to control the cellular translational capacity and that during heat stress the raised cellular levels of the alarmone were able to curb the rate of protein synthesis. Furthermore, (p)ppGpp controls the heat-induced expression of Hpf and thus the formation of translationally inactive 100S disomes. These results indicate that B. subtilis cells respond to heat-mediated protein unfolding and aggregation, not only by raising the cellular repair capacity, but also by decreasing translation involving (p)ppGpp mediated stringent response to concurrently reduce the protein load for the cellular protein quality control system.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:57:26.067.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Frese
SpeciesList scientific name: Bacillus subtilis GB03; NCBI TaxID: 1423687;
ModificationListmonohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-11 23:31:20ID requested
12020-03-09 07:23:57announced
22020-06-12 02:37:40announced2020-06-12: Updated publication reference for PubMed record(s): 32176689.
32024-10-22 04:57:34announced2024-10-22: Updated project metadata.
Publication List
Sch, ä, fer H, Beckert B, Frese CK, Steinchen W, Nuss AM, Beckstette M, Hantke I, Driller K, Sudzinov, á P, Kr, á, sn, ý L, Kaever V, Dersch P, Bange G, Wilson DN, Turgay K, The alarmones (p)ppGpp are part of the heat shock response of Bacillus subtilis. PLoS Genet, 16(3):e1008275(2020) [pubmed]
10.1371/journal.pgen.1008275;
Keyword List
curator keyword: Biological
submitter keyword: Bacillus subtilis
Heat stress response
stringent response
(p)ppGpp
alarmone
Rel
Spx
Contact List
Christian K. Frese
contact affiliationMax Planck Unit for the Science of Pathogens, Chariteplatz 1, 10117 Berlin, Germany
contact emailfrese@mpiib-berlin.mpg.de
lab head
Christian Frese
contact affiliationMax Planck Unit for the Science of Pathogens
contact emailfrese@mpusp.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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