PXD015414 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | : Hypusine Biosynthesis in β Cells Links Polyamine Metabolism to Facultative Cellular Proliferation to Maintain Glucose Homeostasis |
Description | : Deoxyhypusine synthase (DHPS) utilizes the polyamine spermidine to catalyze the hypusine modification of the mRNA translation factor eIF5A and promotes oncogenesis through poorly-defined mechanisms. Because germline deletion of Dhps is embryonically lethal, its role in normal postnatal cellular function in vivo remains unknown. We generated a mouse model that allows for inducible, postnatal deletion of Dhps specifically in postnatal islet β cells, which function to maintain glucose homeostasis. Removal of Dhps did not have an effect under normal physiologic conditions. However, upon development of insulin resistance, which induces β-cell proliferation, Dhps deletion caused alterations in proteins required for mRNA translation, reduced production of the cell cycle molecule Cyclin D2, impaired β-cell proliferation, and overt diabetes. We found that hypusine biosynthesis was downstream of protein kinase C-ζ and was required for c-Myc-induced proliferation. Our studies reveal a requirement for DHPS in β cells to link polyamines to mRNA translation to effect facultative cellular proliferation and glucose homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-08 |
AnnouncementXML | Submission_2021-09-08_11:54:00.503.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD015414 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Emma Doud |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-11 08:21:34 | ID requested | |
⏵ 1 | 2021-09-08 11:54:01 | announced | |
Publication List
Levasseur EM, Yamada K, Pi, ñ, eros AR, Wu W, Syed F, Orr KS, Anderson-Baucum E, Mastracci TL, Maier B, Mosley AL, Liu Y, Bernal-Mizrachi E, Alonso LC, Scott D, Garcia-Oca, ñ, a A, Tersey SA, Mirmira RG, cells links polyamine metabolism to facultative cellular proliferation to maintain glucose homeostasis. Sci Signal, 12(610):(2019) [pubmed] |
Keyword List
submitter keyword: β cell, proliferation, hypusine, polyamines |
Contact List
Amber Mosley |
contact affiliation | Director of Proteomics Core |
contact email | almosley@iu.edu |
lab head | |
Emma Doud |
contact affiliation | Indiana University School of Medicine |
contact email | edoud@iu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015414
- Label: PRIDE project
- Name: : Hypusine Biosynthesis in β Cells Links Polyamine Metabolism to Facultative Cellular Proliferation to Maintain Glucose Homeostasis