PXD015410

PXD015410 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells revealed an unexpected deviation between 2D monolayer and 3D spheroid model cultures.
Description	Recently, it has been proposed to employ three-dimensional cultivation of cell in spheroids that contributes more proper physiological conditions in matrix extracellular space architecture and cell morphology accompanied by appropriate intracellular biomechanical organization of cytoskeleton and energy generation apparatus adaptation. In this research we presented the data of proteome survey for cells growing in monolayer and in spheroids that were induced in myogenic way of differentiation and reached terminal stage in seven days. Semi-quantitative profiling of the dynamic changes in proteins abundance depicted strict advantage of spheroid manner cultivation in contrast to monolayer. Cells cultured in spheroid were significantly enriched in proteins of mitochondria biogenesis, respiratory chain proteins, extracellular proteins and cytoskeleton. Most of signal transducers involved in active proliferation and early stages of differentiation were inhibited in spheroid cultures unlike monolayer. The obtained data were broadly consistent with known molecular mechanisms of myogenesis in transcripts level and reflected strong advantage of spheroid cultivation conditions.
HostingRepository	PRIDE
AnnounceDate	2021-11-15
AnnouncementXML	Submission_2021-11-15_13:45:50.010.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015410
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arthur Kopylov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Glu->pyro-Glu; Deamidated; Oxidation; Acetyl; Pyridylethyl; Gln->pyro-Glu
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-11 03:36:41	ID requested
⏵ 1	2021-11-15 13:45:50	announced

Saburina IN, Kosheleva NV, Kopylov AT, Lipina TV, Krasina ME, Zurina IM, Gorkun AA, Girina SS, Pulin AA, Kaysheva AL, Morozov SG, Proteomic and electron microscopy study of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells in three-dimensional culture. Proteomics, 22(3):e2000304(2022) [pubmed]

submitter keyword: myogenesis

monolayer culture

spheroid culture

cytoskeleton

mitochondria

autophagy;

Pulin Andrey
contact affiliation	Laboratory for Cell Biology and Developmental Pathology, Institute of General Pathology and Pathophysiology, 8 Baltiyskaya st., Moscow, 125315
contact email	andreypulin@gmail.com
lab head
Arthur Kopylov
contact affiliation	Institute of Biomedical Chemistry
contact email	a.t.kopylov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD015410

PRIDE project URI

• PRIDE
  1. PXD015410
     1. Label: PRIDE project
     2. Name: Proteome profiling of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells revealed an unexpected deviation between 2D monolayer and 3D spheroid model cultures.