PXD015409

PXD015409 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Development of a maleimide-based chemical proteomic method for quantitative analysis of cysteine reactivity
Description	Cysteine is the most intrinsically nucleophilic residue in proteins and serves as a sentinel against increasing reactive oxygen species (ROS) via reversible thiol oxidation. Despite the importance of Cys oxidation in understanding biological stress response, it remains largely unknown and a major analytical challenge to determine which protein Cys-sites are the most reactive toward ROS. Herein, we describe initial coverage and sensitivity of a chemical proteomic method to quantify site-specific Cys reactivity using a maleimide-activated, thiol-specific probe (N-propargylmaleimide, NPM).
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:31:25.334.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015409
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Evan McConnell
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-11 03:15:01	ID requested
⏵ 1	2024-10-07 10:31:25	announced

Publication List

10.6019/PXD015409;

10.6019/PXD015409;

Keyword List

submitter keyword: thiol-reactivity profiling,Chlamydomonas reinhardtii, chemical proteomics, cysteine oxidation

submitter keyword: thiol-reactivity profiling,Chlamydomonas reinhardtii, chemical proteomics, cysteine oxidation

Contact List

Leslie M. Hicks
contact affiliation	Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
contact email	lmhicks@unc.edu
lab head
Evan McConnell
contact affiliation	Graduate Research Assistant
contact email	evanmcc@live.unc.edu
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD015409

PRIDE project URI

Repository Record List

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