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PXD015409

PXD015409 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDevelopment of a maleimide-based chemical proteomic method for quantitative analysis of cysteine reactivity
DescriptionCysteine is the most intrinsically nucleophilic residue in proteins and serves as a sentinel against increasing reactive oxygen species (ROS) via reversible thiol oxidation. Despite the importance of Cys oxidation in understanding biological stress response, it remains largely unknown and a major analytical challenge to determine which protein Cys-sites are the most reactive toward ROS. Herein, we describe initial coverage and sensitivity of a chemical proteomic method to quantify site-specific Cys reactivity using a maleimide-activated, thiol-specific probe (N-propargylmaleimide, NPM).
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:31:25.334.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD015409
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEvan McConnell
SpeciesList scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-11 03:15:01ID requested
12024-10-07 10:31:25announced
Publication List
10.6019/PXD015409;
Keyword List
submitter keyword: thiol-reactivity profiling,Chlamydomonas reinhardtii, chemical proteomics, cysteine oxidation
Contact List
Leslie M. Hicks
contact affiliationDepartment of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
contact emaillmhicks@unc.edu
lab head
Evan McConnell
contact affiliationGraduate Research Assistant
contact emailevanmcc@live.unc.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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