PXD015409 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Development of a maleimide-based chemical proteomic method for quantitative analysis of cysteine reactivity |
Description | Cysteine is the most intrinsically nucleophilic residue in proteins and serves as a sentinel against increasing reactive oxygen species (ROS) via reversible thiol oxidation. Despite the importance of Cys oxidation in understanding biological stress response, it remains largely unknown and a major analytical challenge to determine which protein Cys-sites are the most reactive toward ROS. Herein, we describe initial coverage and sensitivity of a chemical proteomic method to quantify site-specific Cys reactivity using a maleimide-activated, thiol-specific probe (N-propargylmaleimide, NPM). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:31:25.334.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD015409 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Evan McConnell |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | Oxidation; Acetyl; Carbamidomethyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-11 03:15:01 | ID requested | |
⏵ 1 | 2024-10-07 10:31:25 | announced | |
Publication List
Keyword List
submitter keyword: thiol-reactivity profiling,Chlamydomonas reinhardtii, chemical proteomics, cysteine oxidation |
Contact List
Leslie M. Hicks |
contact affiliation | Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA |
contact email | lmhicks@unc.edu |
lab head | |
Evan McConnell |
contact affiliation | Graduate Research Assistant |
contact email | evanmcc@live.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015409
- Label: PRIDE project
- Name: Development of a maleimide-based chemical proteomic method for quantitative analysis of cysteine reactivity