PXD015408

PXD015408 is an original dataset announced via ProteomeXchange.

Title	The pancreatic islet MHC-II peptidome identifies key features of autoimmune peptides
Description	The nature of autoantigens that trigger autoimmune diseases has been much discussed but direct biochemical identification is lacking for most. Addressing this question demands unbiased examination of the self-peptides displayed by a defined autoimmune MHC-II molecule. We have examined the immunopeptidome of the pancreatic islets of the NOD mouse that spontaneously develops autoimmune diabetes based on its MHC-II, the I-Ag7 variant. The relevant peptides that induced early CD4 T cells derived from insulin and C-peptide; these were also found in the MHC-II peptidome of the pancreatic lymph node and spleen. The insulin-derived peptides followed a trajectory from internal processing in beta cells to exocytosis, uptake, and presentation in islets and peripheral sites. Such process not only generated conventional epitopes but also resulted in presentation of post-translationally modified peptides, including deamidated and fused sequences. These analyses reveal the key features of a restricted component in the self-MHC-II peptidome causing autoreactivity.
HostingRepository	PRIDE
AnnounceDate	2020-03-12
AnnouncementXML	Submission_2020-03-12_03:30:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015408
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cheryl Lichti
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Trioxidation; Deamidated; Oxidation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-11 01:33:52	ID requested
⏵ 1	2020-03-12 03:30:09	announced

Wan X, Vomund AN, Peterson OJ, Chervonsky AV, Lichti CF, Unanue ER, The MHC-II peptidome of pancreatic islets identifies key features of autoimmune peptides. Nat Immunol, 21(4):455-463(2020) [pubmed]

submitter keyword: NOD mouse, MHC-II peptidome, spleen, pLN, islets, hybrid insulin peptides

Cheryl Lichti
contact affiliation	Department of Pathology & Immunology, Washington University, St. Louis, MO 63110
contact email	clichti@wustl.edu
lab head
Cheryl Lichti
contact affiliation	Washington University
contact email	clichti@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015408
     1. Label: PRIDE project
     2. Name: The pancreatic islet MHC-II peptidome identifies key features of autoimmune peptides