PXD015403

PXD015403 is an original dataset announced via ProteomeXchange.

Title	Environmental viscosity modulates interbacterial killing during habitat transition
Description	In this study we employed a proteomics method to determine how bacteria respond at the protein level to a transition from a low- to a high-viscosity liquid environment. We applied this method to explore how the symbiotic marine bacterium Vibrio fischeri responds to changes in environmental viscosity associated with the transition from free-living to host-associated states. For this project cultures of ES401 were initially grown in low-viscosity liquid media and then transferred to either fresh low- or high-viscosity liquid media, which was generated by supplementing liquid media with 5% polyvinylpyrrolidone (w/vol). Samples were then incubated for one, 12, or 24 hours, and then collected by centrifugation and cell pellets frozen at -80°C. For each treatment four biological replicates were generated and analyzed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:42.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernanda Salvato
SpeciesList	scientific name: Aliivibrio fischeri; NCBI TaxID: 668;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-11 01:15:08	ID requested
1	2020-01-07 09:39:12	announced
2	2020-02-17 01:37:31	announced	2020-02-17: Updated publication reference for PubMed record(s): 32019799.
3	2020-02-27 05:41:23	announced	2020-02-17: Updated publication reference for PubMed record(s): 32019799. 2020-02-27: Updated publication reference for PubMed record(s): 32019799.
⏵ 4	2024-10-22 04:58:50	announced	2024-10-22: Updated project metadata.

10.1128/mBio.03060-19;

Speare L, Smith S, Salvato F, Kleiner M, Septer AN, Environmental Viscosity Modulates Interbacterial Killing during Habitat Transition. mBio, 11(1):(2020) [pubmed]

submitter keyword: Aliivibrio fischeri, Squid-Vibrio symbiosis, Vibrio fischeri, symbiont,1D-LC-MS/MS, Type VI secretion system

Manuel kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Fernanda Salvato
contact affiliation	North Carolina State University
contact email	fersalvato@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015403

PRIDE project URI

• PRIDE
  1. PXD015403
     1. Label: PRIDE project
     2. Name: Environmental viscosity modulates interbacterial killing during habitat transition